Determine protein concentration based on the following absorbance: 0.42 and 0.54 Y= 0.3958x R^2= 0.9778
Homework Answers
y=0.3958x x is the protein concetration on x axis., y is the absorbance.
for,0.42,
0.42=0.3958 x, so x=1.061 unit
for,0.54,
0.54 =0.3958 x, so x=1.364 unit
Add Answer to:
Determine protein concentration based on the following absorbance:
0.42 and 0.54
Y= 0.3958x
R^2= 0.9778
please answer 1 and 2 table 4 protein concentrations 1. Determine the protein concentrations for your...
please answer 1 and 2
table
4
protein
concentrations
1. Determine the protein concentrations for your positive and negative control and your test sample (Table 4) using the protein standard curve you constructed. Include Table 4 and your protein concentrations in your response. Do these results seem accurate to you? Did they differ from what you expected for the food item you brought into class? 2. What is the R2 value of your known protein concentrations plotted against their absorbency?...
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Using the equation y=0.0165x in the figure below, where y= absorbance and x= concentration in mmol/L,...
Using the equation y=0.0165x in the figure below, where y=
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please answer 1 and 2
table
4
protein
concentrations
1. Determine the protein concentrations for your positive and negative control and your test sample (Table 4) using the protein standard curve you constructed. Include Table 4 and your protein concentrations in your response. Do these results seem accurate to you? Did they differ from what you expected for the food item you brought into class? 2. What is the R2 value of your known protein concentrations plotted against their absorbency?...
Using the equation y=0.0165x in the figure below, where y=
absorbance and x= concentration in mmol/L, calculate the
concentraion of Cu2+ in a sample whose absorbance is 0.275. Show
your work, and include correct unit for concentration
0.350 0.300 0.250 y = 0.0165x R = 0.9983 unk 0 U 0.200 0.150 0.100 0.050 0.000 0.0 5.0 10.0 15.0 Cunk 20.0 25.0 Concentration of Cu2+ standards, mmol/L
Need a graph with the absorbance and the concentration plotted with
the x and y axis the unknown is .508 absorbance and the the unknown
concentration is 24.19 . With these values beers gas laws
15 EXPERIMENT 45 Report Sheet 1. Unknown number 2. Data table Tube # | Dye olume (mL) | DyeconcentrationhW | Ahorhance (520mm, 0 c00 20 32698 | 85-1 | :779 :132 46.8 3. Unknown absorbance value 4 Uniknown concentration from the graph
A 1.00 liter solution contains 0.54 moles nitrous acid and 0.42 moles potassium nitrite . If 0.21 moles of perchloric acid are added to this system, indicate whether the following statements are true or false. (Assume that the volume does not change upon the addition of perchloric acid.) A. The number of moles of HNO2 will remain the same. B. The number of moles of NO2 will increase. C. The equilibrium concentration of Hz0+ will increase. D. The pH will...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
If the molar absorptivity at a specific wavelength is known for a protein, it is pretty easy to determine its concentration simply by measuring its absorbance and using Beers law. Based on the following information, spectrophotometrically determine the concentration (in mg/mL) of a solution of the protein lysozyme. -the molar absorptivity at 280 nm (converted for units of mg/mL) is & = 0.243 mg.cm -the absorbance of a solution of lysozyme is 0.923 when the path length is 1 cm.
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