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6. What physical characteristics of a biomolecule influence its rate of movement in an electrophoresis matrix? 7. Design a diagnostic test based on Western blot that would give an indication of infection by the AIDS virus. Assume the blood serum sample is available from the patient.
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Answer 6.Factors affecting electrophoretic movement of molecules are:

1. Charge: there is increase in rate of movement with increase in net charge. Net charge sometimes affected by pH of electrophoresis matrix.

2. Size: large molecules move slowly and small molecules move fast. Large molecules will be having more friction and electrostatic interaction than small so their speed will be slow.

3. Shape: Molecules with same charge and size but having different shape shows different rate of movement.

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