Homework Answers
Add Answer to:
How many recombinant DNA molecules of 60 kb size would you need to clone for a...
The human genome contains about 3x10^9 bp of DNA. How many 200-kb fragments would you have...
The human genome contains about 3x10^9 bp of DNA. How many 200-kb fragments would you have to clone into a BAC library to have a 90% probability of in- cluding a particular sequence?
Assume that a cosmid will carry inserts of about 50 kb and that cosmids are used...
Assume that a cosmid will carry inserts of about 50 kb and that cosmids are used to clone a 3 Mb (megabase: 1 Mb = 1,000 Kb) genome. Assuming you are particularly lucky and have no duplication in your library, what is the smallest number of cosmid clones you would need for a "complete" genomic library? 1 3000 clones 2) 600 clones 3) 300 clones 4 30 clones ⓢ 60 clones
How many nucleotides long would a DNA sequence need to be in order for it not...
How many nucleotides long would a DNA sequence need to be in order for it not to be found by chance more than once in a human genome (size is 3.2 x 10^9 base pairs)? Set up the correct calculation below - SHOW YOUR WORK!
How can you use PCR in the process of generating recombinant DNA. Why would we need...
How can you use PCR in the process of generating recombinant DNA. Why would we need to add a restriction enzyme site onto a fragment of interest in the process of generating recombinant DNA? Why would you benefit from adding two different enzyme sites to a given fragment?
A piece of DNA that is 3 kb in length is found in many locations throughout...
A piece of DNA that is 3 kb in length is found in many locations throughout the genome. How would you decide whether this was a LINE or a DNA transposon? a. If it is transcribed, then it must be a LINE; if not, then it is a DNA transposon. b. If it has more than 10,000 copies in the genome, it is a LINE; if not, it is a DNA transposon. c. If it uses an RNA intermediate, it...
BTH2732 Recombinant DNA Technology For answer all questions below part, Ignore question 1. Just do question...
BTH2732 Recombinant DNA Technology
For answer all questions below part, Ignore question 1. Just
do question 2 and below
Part A: Refer to Lecture 3 & Supplementary Video: https://www.youtube.com/watch?v SiwNtQYLKeU Virtual cloning of the Human p53 gene You are required to devise a set of practical methodologies in order to carry out a relatively simple molecular biology task. 1. You must identify the steps required 2. Formulate a set of practical protocols needed to carry out those steps . Consider...
You have isolated a strain of brewer's yeast (Saccharomyces cerevisiae) in the lab for your second...
You have isolated a strain of brewer's yeast (Saccharomyces cerevisiae) in the lab for your second job at a hip new microbrew (you make a great IPA). You find that this strain ferments more efficiently and adds a superior flavour profile to your brews. You want to clone the strain of yeast and generate a genomic library to determine the genes responsible for this finding. To do so you: 1. Obtain fragments of the whole yeast genome (DNA) through restriction...
please explain 15. If you are amplifying a target sequence of 3.0 kb from a genomic...
please explain
15. If you are amplifying a target sequence of 3.0 kb from a genomic DNA sample of size 3.0 x 10 kb, after 20 rounds of PCR what percentage of the total DNA would be your target sequence? 3.0-10 C. f= 220 X3.0 2 20 X3.0 B. f 220X3.0+3.0-1051.2% 2 30 =95.4% 104.9% A. f X3.0 3.0x10 16. The differences in the human genome between individuals are called B. genetic micro-satellites (GMS) D. minor allele frequency (MAF) A....
In a hypothetical scenario. You wake up one morning to find your hah has suddenly grown....
In a hypothetical scenario. You wake up one morning to find your hah has suddenly grown. YOU have been supplementing your diet with a strange now fungus purchased at the local farmer's market. You take samples of the fungus to your lab and you find that this fungus does indeed make a protein (the her E protein) that stimulates hair growth. Yon con struct a fungal genomic DNA library in E. Coli with the hope of cloning the hart gene....
How many actual crossovers are represented by each recombinant Sordaria ascus? What would happen if the...
How many actual crossovers are represented by each recombinant Sordaria ascus? What would happen if the other two chromatids also crossed over? Would you be able to tell if this happened when examining your squashes?
Assume that a cosmid will carry inserts of about 50 kb and that cosmids are used to clone a 3 Mb (megabase: 1 Mb = 1,000 Kb) genome. Assuming you are particularly lucky and have no duplication in your library, what is the smallest number of cosmid clones you would need for a "complete" genomic library? 1 3000 clones 2) 600 clones 3) 300 clones 4 30 clones ⓢ 60 clones
BTH2732 Recombinant DNA Technology
For answer all questions below part, Ignore question 1. Just
do question 2 and below
Part A: Refer to Lecture 3 & Supplementary Video: https://www.youtube.com/watch?v SiwNtQYLKeU Virtual cloning of the Human p53 gene You are required to devise a set of practical methodologies in order to carry out a relatively simple molecular biology task. 1. You must identify the steps required 2. Formulate a set of practical protocols needed to carry out those steps . Consider...
You have isolated a strain of brewer's yeast (Saccharomyces cerevisiae) in the lab for your second job at a hip new microbrew (you make a great IPA). You find that this strain ferments more efficiently and adds a superior flavour profile to your brews. You want to clone the strain of yeast and generate a genomic library to determine the genes responsible for this finding. To do so you: 1. Obtain fragments of the whole yeast genome (DNA) through restriction...
please explain
15. If you are amplifying a target sequence of 3.0 kb from a genomic DNA sample of size 3.0 x 10 kb, after 20 rounds of PCR what percentage of the total DNA would be your target sequence? 3.0-10 C. f= 220 X3.0 2 20 X3.0 B. f 220X3.0+3.0-1051.2% 2 30 =95.4% 104.9% A. f X3.0 3.0x10 16. The differences in the human genome between individuals are called B. genetic micro-satellites (GMS) D. minor allele frequency (MAF) A....
In a hypothetical scenario. You wake up one morning to find your hah has suddenly grown. YOU have been supplementing your diet with a strange now fungus purchased at the local farmer's market. You take samples of the fungus to your lab and you find that this fungus does indeed make a protein (the her E protein) that stimulates hair growth. Yon con struct a fungal genomic DNA library in E. Coli with the hope of cloning the hart gene....
Most questions answered within 3 hours.
-
A gas occupies 200. mL in a piston. If the pressure of the
piston were decreased...
asked 16 minutes ago -
A fossil is found to have a 14C level of 71.0% compared to
living organisms. How...
asked 20 minutes ago -
Many communist or socialist countries have a department that
addresses public health as well as the...
asked 22 minutes ago -
the following questions are either true or false answers
1. The Central Limit Theorem allows one...
asked 22 minutes ago -
The patient recovery time from a particular surgical procedure
is normally distributed with a mean of...
asked 28 minutes ago -
Human relations refer to the way a company arranges people,
jobs, and communications so that work...
asked 45 minutes ago -
Python Program: Design the logic for and implement a program
that merges the two files into...
asked 43 minutes ago -
The specific radiocarbon activity of a sample of wood is 6.25
gms dpm/gm of carbon. The...
asked 48 minutes ago -
An aqueous magnesium chloride solution is made by dissolving
6.96 moles of MgCl2 in sufficient water...
asked 51 minutes ago -
Ken believes the average age of men who come to get a haircut at
his barber...
asked 1 hour ago -
(Ratio Analysis): Last year Co. XYZ had sales of $ 400,000, with
“cost of goods sold”...
asked 1 hour ago -
can someone please write the balanced chemical
equation for the synthesis of Bromoacetanilide
from;
aniline +...
asked 1 hour ago