The human genome contains about 3x10^9 bp of DNA. How many 200-kb fragments would you have...
The human genome contains about 3x10^9 bp of DNA. How many 200-kb fragments would you have to clone into a BAC library to have a 90% probability of in- cluding a particular sequence?
Homework Answers
Size of genome = 3 x 109 bp
= 3 x 109 / 1000
= 3 x 106 kb
Number of 200 kb fragments to be cloned into BAC library = 3 x 106 kb / 200 kb
= 15000 fragments
A total of 15 x 103 fragments of the size 200 kb can be cloned into BAC library from the human genome.
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