you have heard about three different vectors to clone DNA fragments (plasmids, BACs, and YACs). Which of these would be most appropriate to clone a DNA fragment with the length of 5,000 bp, and which would be most appropriate for a fragment of 200,000 bp?
The genome of fruit flies has a size of about 175 Mbp. Assuming that each base pair has a height of 3 Angstrom (1 Angstrom is 10^-10 m), how long is the genome of a fruit fly in meters / millimeters?
Q.1
Answer:
Explanation:
Maximum insert size limit:
Plasmid = 10kb (10,000 bp)
BAC = 300 Kb (300,000 bp)
YAC = 1400 Kb (1,400,000 bp)
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Q.2
Solution:
175 Mbp = 175,000 Kbp
175,000 Kbp = 175,000,000 bp
> 175,000,000 * 3 = 525,000,000 Angstrom
> 525,000,000 * 10^-10 m = 0.0525 m
Answer: 0.0525 meter
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