Question

QUESTION 31 You have a DNA sequence of 1600 bp. To characterize it, you decide to make a restriction map. Cutting with the first enzyme, you show by electrophoresis that the fragments produced are 400 and 1200 bp, respectively. After using the second enzyme, you recover 700-bp and 900-bp fragments. After treatment with both enzymes, fragments were 300, 400, and 900 bp. Which is the correct map? O -400/--900----/300 --- -300-/--400--/--900---- O-900 ------/400--/3-- -400-/300----900--- -400-/-100/--300--/900 QUESTION 32 Which of the following would be found in a cDNA library, but not a genomic library? introns replication origins centromeres exons promoters QUESTION 33 What is the role of Case in the CRISPR-Cas9 gene-editing system? a. Helps the cell to recognize and repair damaged DNA b. Unes DNA repair machinery to introduce changes to genes c. Ensures the Case enzyme cuts at the correct sequence Click Save and Submit to save and submit. Click Save All Answers to save all answers MacBook Pro

Answer 1

31) answer is 4th option

1600 kb upon digestion with 1st enzyme produces 400 and 1200

On digestion with 2nd enzyme produces 700 bp and 900 bp.

It's obvious that then upon double digestion, it produces in the order 400, 300&900 kbs  

32) exons

Because cDNA library will only contain sequences in mRNA

On the other hand,In genomic DNA library they contains both sequences, ie, intron and exon sequences