Homework Answers
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32,...
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32, and 1:64, there is no visible agglutination in the 1:64 tube. What is the titer of the antibody? A. 32 B. 16 C. 64 D. Cannot be determined 2. If you want to make 2 mL of a 1:20 dilution, how much serum would be needed? 3. When 1.0 mL of serum is added to 3.0 mL of diluent, what dilution does this represent?...
17. One milliliter of plasma is diluted 1/10 in tube 1. Then 1 mL of the...
17. One milliliter of plasma is diluted 1/10 in tube 1. Then 1 mL of the dilution in tube 1 is added to 4 mL in a second tube. One milliliter from tube 2 is then added to 9 mL diluent in tube 3. What is the substance (plasma) volume in the third tube? 18. What is the specific gravity of a substance if 5 mL of the substance weighs 6.5 g, but the substance is only 50% pure? 19.What...
Question 1 20 pts Please prepare 20 mL of 0.2 M NaOH, given a stock solution of 0.6 M NaOH. State...
Question 1 20 pts Please prepare 20 mL of 0.2 M NaOH, given a stock solution of 0.6 M NaOH. State the precise amount of the 0.6 M NaOH used and the amount of diluent (H2O) added Question 2 20 pts Please prepare 500 mL of a 1/100 dilution of serum from a tube with a 1/10O dilution of serum. Please state the volume of the 1/10 dilution of serum used and the volume of diluents (H2O) used 20 pts...
dilutions dilutions What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L...
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased...
1) How would you prepare 500mL of working TBE buffer if the stock solution was purchased as 25X TBE? 2) 2mL of a serum sample was added to 18mL of phosphate buffered saline (PBS) in Tube 1. 10mL of Tube 1 was added to 40mL of PBS in Tube What is the dilution of serum in Tube 2? 3) A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab...
You are setting up an experiment to first obtain the line of best fit using inorganic phosphate s...
You are setting up an experiment to first obtain the line of best fit using inorganic phosphate standards. Calculate the amount of phosphate in each tube (shaded in blue). Standard concentration is 1 μmol/mL phosphate. (2 marks) Reagent blank Standards Unknown Tube no. 1 2 3 4 5 6 7 89 10 11 12 13 14 Volume of std00 0.2 0.2 0.4 0.4 0.6 0.6 0.8 0.8 1.0 1.0 phosphate (mL) (mL) Amount of phosphate Volume of unknown 0 00...
phenol is in each of your unknowns. 1. You will be given a nitrophenol solution of...
phenol is in each of your unknowns. 1. You will be given a nitrophenol solution of concentration 400 nmoles/mL and you will use this stock solution to make a serial dilution as follows: To make the serial dilution you need to prepare 5 tubes with 1 ml water. Next, add 1ml from the 400 nmoles/ml stock to the first tube. Pipette the solution up and down carefully and remove 1 ml of this solution to the next tube. Repeat this...
Answer the following questions regarding a 10x Serial Dilution experiment using yeast cell cultures from tube...
Answer the following questions regarding a 10x Serial Dilution experiment using yeast cell cultures from tube A. Tube A contains 1 ml of yeast culture. 3.6 ml of sterile water is placed into 4 tubes (B, C, D, and E). 0.4 ml of yeast culture is removed from tube A and put into tube B.. 0.4 ml is transferred from tube B to tube C, and from tube C to tube E. 1.) What is the final volume in tubes...
In order to count cells, the culture must be duted The technique o serialul precise and...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the...
(A) You have a sample with an original concentration of 1.0 x 10^7 CFU/mL. With the Plate Count Method, what final dilution factor would be needed to produce countable plates? Show your work. (B) Describe a dilution scheme (how many tubes, what volume in each tube, what DF is achieved in each step) that uses only the 9-mL blank diluent tubes to achieve the dilution needed for this FDF.
1. On a serial dilution with the following tubes set up, 1:2, 1:4, 1:8, 1:16, 1:32, and 1:64, there is no visible agglutination in the 1:64 tube. What is the titer of the antibody? A. 32 B. 16 C. 64 D. Cannot be determined 2. If you want to make 2 mL of a 1:20 dilution, how much serum would be needed? 3. When 1.0 mL of serum is added to 3.0 mL of diluent, what dilution does this represent?...
17. One milliliter of plasma is diluted 1/10 in tube 1. Then 1 mL of the dilution in tube 1 is added to 4 mL in a second tube. One milliliter from tube 2 is then added to 9 mL diluent in tube 3. What is the substance (plasma) volume in the third tube? 18. What is the specific gravity of a substance if 5 mL of the substance weighs 6.5 g, but the substance is only 50% pure? 19.What...
Question 1 20 pts Please prepare 20 mL of 0.2 M NaOH, given a stock solution of 0.6 M NaOH. State the precise amount of the 0.6 M NaOH used and the amount of diluent (H2O) added Question 2 20 pts Please prepare 500 mL of a 1/100 dilution of serum from a tube with a 1/10O dilution of serum. Please state the volume of the 1/10 dilution of serum used and the volume of diluents (H2O) used 20 pts...
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
You are setting up an experiment to first obtain the line of best fit using inorganic phosphate standards. Calculate the amount of phosphate in each tube (shaded in blue). Standard concentration is 1 μmol/mL phosphate. (2 marks) Reagent blank Standards Unknown Tube no. 1 2 3 4 5 6 7 89 10 11 12 13 14 Volume of std00 0.2 0.2 0.4 0.4 0.6 0.6 0.8 0.8 1.0 1.0 phosphate (mL) (mL) Amount of phosphate Volume of unknown 0 00...
phenol is in each of your unknowns. 1. You will be given a nitrophenol solution of concentration 400 nmoles/mL and you will use this stock solution to make a serial dilution as follows: To make the serial dilution you need to prepare 5 tubes with 1 ml water. Next, add 1ml from the 400 nmoles/ml stock to the first tube. Pipette the solution up and down carefully and remove 1 ml of this solution to the next tube. Repeat this...
In order to count cells, the culture must be duted The technique o serialul precise and quantitative method alle e n al b e performed allow you to determine how many well per miller were the time the were made in overview knowvolume of culturedded to know Volume 0 sterile diluent aliquid that will not harm the cell The liquid in this tube is mixed and known volume of this culture is transferred to another known volume of sterile diluent...
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