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You are setting up an experiment to first obtain the line of best fit using inorganic phosphate s...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
How do I make a line of best fit (regression) with the following data? I am...
How do I make a line of best fit (regression) with the
following data? I am aware of how to make it in Microsoft Excel,
but I am having trouble figuring out how to find my x value for
making the graph. Y value is absorbance, what do I use for x value
to calculate the slope?
Tube 0.5 mM sulfide standard (ml) Sulfide amount (umol) Sulfide reagent (ml) 0.0 4.9 0.11 0.00 0.2 FeCl3 dH2O to Absorbance 10ml total...
1- What is a standard curve and how is it used in biochemistry? 2- Explain how...
1- What is a standard curve and how is it used in biochemistry? 2- Explain how the Lambert-Beer equation is applied in this experiment. 3- Why is a spectrophotometer used to obtain data? 4- Based on the experimental data provided, estimate the amount of amino acid in the given unknown solution by Ninhydrin method. SI No. Amount of amino acid [pg) OD at 570nm 1 2 3 4 5 6 Volume of standard amino acid solution (ml) Blank 0.2 0.4...
Principles of Inorganic Chemistry! Week 5 Effect of Temperature on Solubility of a Salt In this experiment, you w...
Principles of Inorganic Chemistry! Week 5 Effect of Temperature on Solubility of a Salt In this experiment, you will study the effect of changing temperature on the amount of solute that will dissolve in a given amount of water. Water solubility is an important physical property in chemistry, and is often expressed as the mass of solute that dissolves in 100 g of water at a certain temperature. In this experiment, you will completely dissolve different quantities of potassium nitrate,...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
How do I make a line of best fit (regression) with the
following data? I am aware of how to make it in Microsoft Excel,
but I am having trouble figuring out how to find my x value for
making the graph. Y value is absorbance, what do I use for x value
to calculate the slope?
Tube 0.5 mM sulfide standard (ml) Sulfide amount (umol) Sulfide reagent (ml) 0.0 4.9 0.11 0.00 0.2 FeCl3 dH2O to Absorbance 10ml total...
1- What is a standard curve and how is it used in biochemistry? 2- Explain how the Lambert-Beer equation is applied in this experiment. 3- Why is a spectrophotometer used to obtain data? 4- Based on the experimental data provided, estimate the amount of amino acid in the given unknown solution by Ninhydrin method. SI No. Amount of amino acid [pg) OD at 570nm 1 2 3 4 5 6 Volume of standard amino acid solution (ml) Blank 0.2 0.4...
Principles of Inorganic Chemistry! Week 5 Effect of Temperature on Solubility of a Salt In this experiment, you will study the effect of changing temperature on the amount of solute that will dissolve in a given amount of water. Water solubility is an important physical property in chemistry, and is often expressed as the mass of solute that dissolves in 100 g of water at a certain temperature. In this experiment, you will completely dissolve different quantities of potassium nitrate,...
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