You want to make 200 ml of PBS with 137mM NaCl, 2.7mM KCL and 8mM Na2HPO4and...
You want to make 200 ml of PBS with 137mM NaCl, 2.7mM KCL and 8mM Na2HPO4and 2mM KH2PO4.solution. You’ve already added the Na2HPO4and theKH2PO4 (whew!) but you still need to add the NaCl and KCl from your stock solutions. Your stock solution of NaCl is 2M and the stock of KCl is 0.5M. How much NaCl and KCl do you need to add?
Homework Answers
|
Component |
Molarity |
|
NaCl (mw: 58.4 g/mol) |
137mM |
|
KCl (mw: 74.551 g/mol) |
2.7mM |
|
Na2HPO4 (mw: 141.96 g/mol) |
8mM |
|
KH2PO4 (mw: 136.086 g/mol) |
2mM |
Stock solution:
|
Components |
Molarity |
|
NaCL |
2M |
|
KCL |
0.5M |
We have to make 200 mL PBS
Use C1/V1= C2/V2
C1V1 = Concentration/amount (start) and Volume (start)
C2V2 = Concentration/amount (final) and Volume (final)
In case of NaCL
C1V1 = C2V2
200mM× x mL =137 mM ×200 mL
x= 137 mM ×200 mL/200mM
x=13.7 mL or NaCL = 13.7 mL
In case of KCL
C1V1 = C2V2
500mM × y mL =2.7 mM ×200 mL
y=2.7 mM ×200 mL/500mM
y=1.08 mL or KCL = 1.08 mL
Adjust solution to desired pH (typically pH ≈ 7.4).
Add Answer to:
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