Question

You need to make a 1:5 dilution of your mitochondria extract in PBS buffer. You want a total volume of your diluted sample to be 50 microliters.

a. what volume of mitochondria extract do you need?

b. what volume of PBS buffer do you need?

Answer 1

total sample = 50 microliters

to get 1:5 dilution ,take 8.33 microliters of mitochondria and add it to 41.65 microliter of PBS buffer.

let us take total 50 micro L = 6 parts

in which one part mitochondria , 5 parts PBS buffer.so that we will get above volumes