Question

Design an experiment to identify a specific protein marker for heart attack risk factor.

1) use patient samples & normal samples
2) Run 2D gel protein electrophoresis
3) Compare the pattern
4) identify the specific marker

Explain the steps in detail.

Answer 1

1) Patient samples and normal samples are used becasue the result can be comprehended with better accuracy when comparing with the normal smples.The concentration of the protein marker as a risk factor may be clearly analysed by using the normal sampple

2)A 2D electrophoresis is important because it identifies the specific proteins from the population with a very high resolution in a single process.The principle involve sepration according to the pI values and also the molecular mass of the proteins.

3)Comparison is done analyse the various properties and nature of the proteins

4)Identification of the specific marker can be done by comparison of the normal sample and the patient sample.The patient sample will show the marker from the normal sample.