3. Specifically describe what would occur in DNA replication if the following components were missing, be thoughtful and do not simply state that DNA replication would not occur. Differentiate between prokaryotic and eukaryotic replication when appropriate.
DNA ligase-
DNA polymerase-
Primer-
Primase-
Topoisomerase-
Helicase-
SSBP-
Sliding clamp/PCNA-
Telomerase-
DNA ligase: Okazaki fragments on the lagging strand cannot be joined together to form a continuous strand.
DNA polymerase: Polymerization of nucleotides to produce a DNA strand in 5'-3' direction does not occur.
Primer: Initiation of replication does not occur as DNA polymerase requires a free 3'-OH group for chain extension.
Primase: RNA primer cannot be synthesized to strat strand extension in the 5'-3' direction.
Topoisomerase: helicase cannot unwind the helix as supercoiling would lead to the breaking of DNA strands.
Helicase: DNA double helix cannot be unwound.
SSBP: The unwound DNA duplex would rewind to form the duplex again.
Sliding clamp/PCNA- Processivity of the polymerase would be reduced.
Telomerase: Chromosomal ends would be deleted in the new strand.
3. Specifically describe what would occur in DNA replication if the following components were missing, be...
5 Describe the process of DNA replication; include the following terms: antiparallel structure, DNA Okazaki fragments, DNA ligase, primer, primase, helicase, topoisomerase, single-strand binding proteins. Describe the function of Helicase, Primase, topoisomerase, DNA Polymerase III, DNA Polymerase I, DNA ligase
DNA Replication - Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means.
QUESTION 10 Below is a list of proteins required for DNA replication to occur and functions involved in DNA replication. Match each protein to its function. helicase A catalyzes phosphodiester bonds between two DNA fragments in a strand single strand binding proteins (SSBPs) Rremoves RNA primer and replaces it with DNA topoisomerase cholds DNA polymerase in place during strand elongation - primase D. breaks hydrogen bonds between base pairs and opens the double helix DNA polymerase in E. extends a...
The enzyme helicase is an important part of the replication machinery, but its use now requires specifically the activity of the following enzyme: Sliding clamp O Topoisomerase Ligase O DNA polymerase O Primase
Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means. If you speak about something you have...
Question 5 1 pts The enzyme helicase is an important part of the replication machinery, but its use now requires specifically the activity of the following enzyme: Topoisomerase ODNA polymerase Sliding clamp Primase Ligase Question 7 1 pts Much of replication can be studied using extracts from wild type (normal) yeast cells that supply all of the essential components. Compared to wild type extracts, if you use an extract that is missing the sliding clamp AND ligase, what do you...
Describe the process of DNA replication. (Your answer should include the following: replication fork, semiconservative replication, replication fork, DNA gyrase, helicase, primase, DNA polymerase, DNA ligase, leading strand, lagging strand, continuous replication, non-continuous replication, and Okazaki fragment)
(Molecular Biology) Define the following components of replication: polymerase, helicase, DNA ligase, RNA primase, and ssDNA binding proteins.
Answer the following genetics questions: a. In detail describe the process of DNA replication. Be sure to include all the major enzymes (gyrase, helicase, ligase, polymerase, primase), SSBs, replication fork, Okazaki fragments, and strand types (leading v. lagging). Draw and label a diagram of a replication bubble and replication forks. b. Describe and differentiate among the primary, secondary, and tertiary structures of a protein. To what kinds of interactions can each of these stages be ascribed? c. What are...
are these correct? I also need help with the blanks - MATCHING (In each blank place the letter of the term in the blank next to appropriate phrase. Use letters only once you might not need all letters) repeats M. Replication - MATCHING (In each blank, the most appropria RNA polymerasel DNA polymerase RNA polymerase Il S→ 3' delta/epsilon RNA polymerase 111 3-5 exonuclease DNA polymerasel semiconservative activity DNA polymerase II short tandem DNA 5'3'exonuclease DNA polymerase III activity DNA...