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The enzyme helicase is an important part of the replication machinery, but its use now requires specifically the activity of
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Answer #1

Answer will be 2 nd option topoisomerae.

Helicase initially cause unwinding of DNA double helical strand locally so that both the strand become seperated and DNA pol can bind and start replication of both the strand. But as the dna pol moves ahead there is poistive supercoiling and it it continous then DNA pol halt, so to overcome this supercoiling problem, topoisomerae also called DNA gyrase come into picture and cut one strand or both the strand and rejoin with respective strand in negative supercoiled way so that it will relax positive supercoiling.

So DNA helicase work along with topoisomerase

Hope it's clear..thanks

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