Question

DNA replication involves the enzyme to unwind the double stranded DNA and enzyme to seal the Okazaki fragments O Polymerase, ligase O Helicase, polymerase Helicase, ligase Ligase, primase Reset Selection Mark for Review What's This?

Answer 1

* Replication in PROKARYOCYTES - It is a semíconservative process + 'Parent DNA has 2 shrands complementary to each other Both stands undergo simultaneous replication to produce two daughter molecules. In this half of the original DNA is conserved in the daughter DNA. * Initiation of DNA synthesis occurs at a site called origin of replication * The separation of a strands of parent DNA results in the foomation a a replication fork- DNA Helicases- These enzymes bind to both the DNA strands at the replication fork. Helicases move along the DNA helix and separate the shands. Their function is comparable with a zip opener. Helicases are dependent on ATP -ox ene sa suPP Okazaki pieces. The small fragments of the discontinuosly Synthesized DNA are called Okazaki pieces- These are produced on the begging stand of DNA Okazaki pieces are later joined to form a continuous strand of DNA DNA polymerase I and DNA ligase are responsible for this process. DNA polymerase I removes the RNA primer and takes its position. DNA polymerase I catalyses the Synthesis (5'-> 3' direction of a fragment of DNA that replaces RNA primer * Enzyme DNA ligase catalyses the formation of a phosphodiester linkage behseen the DNA synthesized by polymerase i and small fragments of DNA produced by DNA polymerase-I This process (nick sealing poocess requires energy provided by breakdown of ATP to AMP and PPi.