Question

Some simple eukaryotic genes can be transcribed and translated by prokaryotes like E. coli. However, some eukaryotic genes cannot be expressed by prokaryotes. Which of the following might be a reason for this failure? A. Prokaryotes are not able to cut out the introns nor are they able to O splice the exons together from the eukaryotic gene. B. the prokaryotic genetic code is sometimes different from eukaryotic code. C. Eukaryotic genes often have unique nucleotide bases which are not found in Prokaryotes OD. Eukaryotic genes can't be transcribed outside of a nucleus Reset Selection

Answer 1

Eukaryotic genes cannot be expressed in prokaryotes because of the following reasons

1)post transcriptional modifications like splicing is absent in prokaryotes and the intronic sequences can contribute to the formation of a very different protein in prokaryotes

2)Codon usage effect :Codon can be read in different systems in different ways i.e,the eukaryotic and prokaryotic system can produce 2 amino acids from the same codon.This can lead to the formation of a non-functional peptide