Question

4.      What is the role of each of the following accessory proteins in replication?

a.   Helicase –

b.   Sliding clamp protein –

c.   RNA primase –

d.   DNA ligase –

Answer 1

Helicase - this enzyme is responsible for uncoiling of double stranded DNA into single stranded formed during DNA replication

RNA primase - this enzyme is responsible for the formation of RNA primer which provides free hydroxyl group to DNA polymerase so that it can add deoxyribonucleotides

Sliding clamp protein - this is a complex of proteins which helps in the sliding of DNA polymerase on template DNA molecule

DNA ligase - this enzyme catalyzes the formation of phosphodiester bond between legs and okazaki fragments and joins them together after DNA replication is completed

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