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Homework Answers
5. C
Helicase requires the activity of primase. Helicase binds to Primase leading to the formation of helicase-primase complex called primosome which leads to the formation of primers .
Helicase unwinds the double stranded DNA and Primase synthesise short RNA primers complementary to single stranded DNA.
Primase activity greatly increases in the presence of helicase.
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Question 5 1 pts The enzyme helicase is an important part of the replication machinery, but...
The enzyme helicase is an important part of the replication machinery, but its use now requires...
The enzyme helicase is an important part of the replication machinery, but its use now requires specifically the activity of the following enzyme: Sliding clamp O Topoisomerase Ligase O DNA polymerase O Primase
DNA Replication - Describe the process of DNA replication in a well-organized manner. Start with the...
DNA Replication - Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means.
Describe the process of DNA replication in a well-organized manner. Start with the helicase and go...
Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means. If you speak about something you have...
Question 6 1 pts You created a mutant form of DNA polymerase in which the 3'to...
Question 6 1 pts You created a mutant form of DNA polymerase in which the 3'to 5'exonuclease (proofreading] function has been destroyed, although its polymerase activity is still fine. Which of the following properties do you expect the mutant polymerase to have? O It will polymerize in both the 5-to-3' direction and the 3-to-5' direction. O It will likely make a new strand of DNA (same length) slower than a normal polymerase. It will be more likely to generate thymidine...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
You created a mutant form of DNA polymerase in which the 3'to 5' exonuclease (proofreading] function...
You created a mutant form of DNA polymerase in which the 3'to 5' exonuclease (proofreading] function has been destroyed, although its polymerase activity is still fine. Which of the following properties do you expect the mutant polymerase to have? It will polymerize in both the 5-to-3' direction and the 3'-to-5' direction. o It will likely make a new strand of DNA (same length) slower than a normal polymerase. It will be more likely to generate thymidine dimers. As it gets...
The type of ATPases associated with the integral binding of ATP as part of the transport...
The type of ATPases associated with the integral binding of ATP as part of the transport process and possessing a conserved domain for the binding of ATP is the V type P type F type ABC type no correct choice listed How many replication forks are formed when an origin of replication is opened? 1 2 3 4 Either C or D and depends whether it is a eukaryotic or prokaryotic cell. What part of the DNA replication process would...
Question 5 (2 points) Describe the Okazaki fragment. These are short fragments made on the lagging...
Question 5 (2 points) Describe the Okazaki fragment. These are short fragments made on the lagging strand. They do not have to be stitched together by ligase. These are long fragments made on the lagging strand. They have to be stitched together by ligase These are short fragments made on the leading strand. They have to be stitched together by ligase. These are short fragments made on the lagging strand. They have to be stitched together by ligase. Question 6...
a-F below list the effects on DNA replication that would be expected in a cell if...
a-F below list the effects on DNA replication that would be
expected in a cell if the gene for one of the following proteins
(i-vi) is mutated and nonfunctional. Write the roman numeral in the
space provided, which corresponds to the expected outcome if that
protein was nonfunctional during attempted DNA replication.
v. DNA ligase vi. DNA sliding clamp A) Primers will be laid down at the origin, but no DNA replication can take place to extend those primers replication...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
The enzyme helicase is an important part of the replication machinery, but its use now requires specifically the activity of the following enzyme: Sliding clamp O Topoisomerase Ligase O DNA polymerase O Primase
Question 6 1 pts You created a mutant form of DNA polymerase in which the 3'to 5'exonuclease (proofreading] function has been destroyed, although its polymerase activity is still fine. Which of the following properties do you expect the mutant polymerase to have? O It will polymerize in both the 5-to-3' direction and the 3-to-5' direction. O It will likely make a new strand of DNA (same length) slower than a normal polymerase. It will be more likely to generate thymidine...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
You created a mutant form of DNA polymerase in which the 3'to 5' exonuclease (proofreading] function has been destroyed, although its polymerase activity is still fine. Which of the following properties do you expect the mutant polymerase to have? It will polymerize in both the 5-to-3' direction and the 3'-to-5' direction. o It will likely make a new strand of DNA (same length) slower than a normal polymerase. It will be more likely to generate thymidine dimers. As it gets...
The type of ATPases associated with the integral binding of ATP as part of the transport process and possessing a conserved domain for the binding of ATP is the V type P type F type ABC type no correct choice listed How many replication forks are formed when an origin of replication is opened? 1 2 3 4 Either C or D and depends whether it is a eukaryotic or prokaryotic cell. What part of the DNA replication process would...
Question 5 (2 points) Describe the Okazaki fragment. These are short fragments made on the lagging strand. They do not have to be stitched together by ligase. These are long fragments made on the lagging strand. They have to be stitched together by ligase These are short fragments made on the leading strand. They have to be stitched together by ligase. These are short fragments made on the lagging strand. They have to be stitched together by ligase. Question 6...
a-F below list the effects on DNA replication that would be
expected in a cell if the gene for one of the following proteins
(i-vi) is mutated and nonfunctional. Write the roman numeral in the
space provided, which corresponds to the expected outcome if that
protein was nonfunctional during attempted DNA replication.
v. DNA ligase vi. DNA sliding clamp A) Primers will be laid down at the origin, but no DNA replication can take place to extend those primers replication...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
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