Question

a-F below list the effects on DNA replication that would be expected in a cell if the gene for one of the following proteins (i-vi) is mutated and nonfunctional. Write the roman numeral in the space provided, which corresponds to the expected outcome if that protein was nonfunctional during attempted DNA replication.

v. DNA ligase vi. DNA sliding clamp A) Primers will be laid down at the origin, but no DNA replication can take place to extend those primers replication of the strand is complete with no missing nucleotides and no RNA DNA polymerase will frequently fall off the DNA template. Although it can rebind B) The newly replicated DNA will remain as individual fragments, even though C) and continue, this will cause DNA replication to slow down tremendously D) The lagging strand will remain as individual fragments which are not covalently linked and which contain both RNA and DNA E) DNA polymerase will stall. Little or no new DNA will be synthesized, as the replication fork fails to progress FNo primers will be laid down at the origin. No DNA replication will occur

Answer 1

IV A : dna polymerase extend the sequence after primers are being added.

V B : ligase fill the gap b/w individual fragments.

VI C : dna sliding clamps keeps dna polymerase processive.

II D : untill nuclease removes rna primer dna pol can't fill the gap and ligase can't seal the nick.

III E : Helicase converts double stranded dna to single strand and thus permits replication fork to move.

I F : primase add rna primer from where replication starts.