Which of the following is NOT used as a reporter gene? The green fluorescent protein (GFP)...
Which of the following is NOT used as a reporter gene?
The green fluorescent protein (GFP) gene
The luciferase gene
The GAL4 transcription factor gene
The beta-glucoronidase gene
The yellow fluorescent protein (YFP) gene
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Which of the following is NOT used as a reporter gene?
The green fluorescent protein (GFP)...
Which of the following is a difference between the use of a reporter gene to study...
Which of the following is a difference between the use of a reporter gene to study gene regulation (ex. promoter deletion mapping with a reporter protein) and using a reporter gene to study protein localization? Select all that apply? o When studying protein localization, a fusion protein is created which consists of both the protein of interest and the reporter protein. D Unlike protein localization studies, when studying gene expression, only the reporter gene is expressed and not the actual...
a) Fluorescent proteins such as green fluorescent protein (GFP) are commonly used in microscopy experiments. Describing...
a) Fluorescent proteins such as green fluorescent protein (GFP) are commonly used in microscopy experiments. Describing the process of fluorescence, what is GFP and what is the main benefit of using fluorescent proteins over other fluorophores? b) Describe how fluorescent proteins can be used to (i) study a protein’s gene expression and (ii) localise proteins of interest within cells? c) The resolution of a standard confocal microscope is approximately 200nm. Describe an experiment that can be used to show whether...
A reporter gene--that is, a gene whose activity is relatively easy to detect--is commonly used to...
A reporter gene--that is, a
gene whose activity is relatively easy to detect--is commonly used
to study promoter activity under a variety of investigative
circumstances. Recombinant DNA technology is used to attach such a
reporter gene to the promoter of interest, after which the
recombinant molecule is inserted by transformation or transfection
into the cell or host organism of choice. Green fluorescent protein
(GFP) from the jellyfish Aequorea victoria fluoresces green when
exposed to blue light. Assume that you wished...
Which of the following is important for the design and use of a reporter GFP fusion...
Which of the following is important for the design and use of a reporter GFP fusion protein plasmid to study the subcellular localization of a protein of interest? Select all the apply. Question 9 options: the STOP codon from the gene of the protein of interest must be removed before insertion into the MCS upstream of the GFP gene. The GFP gene should be downstream of the promoter of the gene of interest. The GFP gene should be in the...
You have constructed a new reporter gene to help you find novel zebrafish mutations. The reporter...
You have constructed a new reporter gene to help you find novel zebrafish mutations. The reporter gene consists of several LEG/TCF binding domains in the enhancer fused to the coding region for green fluorescent protein (GFP). Wild-type embryos that contain this transgene normally express it in the brain and eye primordia and have no developmental defects. You then mutagenize this reporter strain to look for new mutants that affect GFP expression. Which developmental pathway are these mutants likely to be...
D 4. You want to use PGLO to produce red fluorescent protein (RFP) instead of green...
D 4. You want to use PGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein (GFP). To do so, you cut out the GFP gene from PGLO with two restriction enzymes, and replace it with the coding sequence for RFP. Which two restriction enzymes will you choose to cut the RFP gene (out of a different plasmid) prior to ligating it into the PGLO plasmid? Select all that apply EcoRI EcoRV Ndel Psti Nhel D 3. You...
Your friend decides to place Green Fluorescent Protein (GFP) under the control of the promoter from...
Your friend decides to place Green Fluorescent Protein (GFP)
under the control of the promoter from the lacZ gene we discussed
in class. She put this expression plasmid into a bacterium. The
promoter from the lacZ gene is diagrammed to the right.?
2) Your friend decides to place Green Fluorescent Protein (GFP) under the control of the promoter from the lacZ gene we discussed in class. She put this expression plasmid into a bacterium. The promoter from the lacZ gene...
I need #2, 3, and 4 please! Above are bacteria expressing the green fluorescent protein (GFP)...
I need #2, 3, and 4 please!
Above are bacteria expressing the green fluorescent protein (GFP) originally cloned from jelly fish. The GFP gene has been inserted into the lac operon such that when the lac operon is ON, the GFP protein will be expressed. The cells above are from a colony. 1. What does the sentence above mean by "inserted into the lac operon"? Draw a figure that explains that sentence mote S 个ャ epressov inding Polymerus CAP 2....
You want to use PGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein...
You want to use PGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein (GFP). To do so you cut out the GFP gene from PGLO with two restriction enzymes, and replace it with the coding sequence for REP After you successfully created a modified version of the PGLO plasmid with the RFP in place of the GFP you transform this plasmid into E. coli and plate it onto LB plates containing both ampicillin and arabinose. After an...
A. You want to make a translational fusion of protein Z and the yellow fluorescent protein...
A. You want to make a translational fusion of protein Z and the yellow fluorescent protein YFP (Z-YFP) so that expression of the fusion occurs with the same cell-specific pattern as that of protein Z. The protein Z gene has a 2 kb promoter, two 1 kb exons, and one 5 kb intron. The YFP gene is 0.7 kb. Outline with words and sketches your strategy to make the Z-YFP fusion clone using PCR. You already have cells that contain...
Which of the following is a difference between the use of a reporter gene to study gene regulation (ex. promoter deletion mapping with a reporter protein) and using a reporter gene to study protein localization? Select all that apply? o When studying protein localization, a fusion protein is created which consists of both the protein of interest and the reporter protein. D Unlike protein localization studies, when studying gene expression, only the reporter gene is expressed and not the actual...
A reporter gene--that is, a
gene whose activity is relatively easy to detect--is commonly used
to study promoter activity under a variety of investigative
circumstances. Recombinant DNA technology is used to attach such a
reporter gene to the promoter of interest, after which the
recombinant molecule is inserted by transformation or transfection
into the cell or host organism of choice. Green fluorescent protein
(GFP) from the jellyfish Aequorea victoria fluoresces green when
exposed to blue light. Assume that you wished...
D 4. You want to use PGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein (GFP). To do so, you cut out the GFP gene from PGLO with two restriction enzymes, and replace it with the coding sequence for RFP. Which two restriction enzymes will you choose to cut the RFP gene (out of a different plasmid) prior to ligating it into the PGLO plasmid? Select all that apply EcoRI EcoRV Ndel Psti Nhel D 3. You...
Your friend decides to place Green Fluorescent Protein (GFP)
under the control of the promoter from the lacZ gene we discussed
in class. She put this expression plasmid into a bacterium. The
promoter from the lacZ gene is diagrammed to the right.?
2) Your friend decides to place Green Fluorescent Protein (GFP) under the control of the promoter from the lacZ gene we discussed in class. She put this expression plasmid into a bacterium. The promoter from the lacZ gene...
I need #2, 3, and 4 please!
Above are bacteria expressing the green fluorescent protein (GFP) originally cloned from jelly fish. The GFP gene has been inserted into the lac operon such that when the lac operon is ON, the GFP protein will be expressed. The cells above are from a colony. 1. What does the sentence above mean by "inserted into the lac operon"? Draw a figure that explains that sentence mote S 个ャ epressov inding Polymerus CAP 2....
You want to use PGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein (GFP). To do so you cut out the GFP gene from PGLO with two restriction enzymes, and replace it with the coding sequence for REP After you successfully created a modified version of the PGLO plasmid with the RFP in place of the GFP you transform this plasmid into E. coli and plate it onto LB plates containing both ampicillin and arabinose. After an...
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