Question

For the mutation below, (a) characterize the mutation thoroughly, (b) give the amino acid sequence resulting from the mutated strand and, (c) state what effect the mutation may have on the function of the gene product

Mutation 2: 3’ g t c A C A C G T T T A g a c g t t a a a c a t c G C G A A G T T T 5’

(a) characterize the mutation thoroughly (6 points)

(b) give the amino acid sequence resulting from the mutated strand (6 points)

(c) state what effect the mutation may have on the function of the original gene product (8 points)

Answer 1

a. The mutation is in the template strand which is used for synthesis of mRNA. Since the direction of the strand is 3'-5' we can say that this is a part of the template strand. An insertion of a single nucleotide has occurred.

b. 3’    g t c   A C A C G T T T A g a c g t t a a a c a t c G C G A A G T T T 5’

mRNA: 5' - C A G UG U G C A A A U C UG C A A UUU G U A G C G C UU C AA A

PROTEIN: Gln - Cys - Ala - Asn - Leu - Gln - Phe- Val - Ala - Leu- Gln

c. frameshift mutation will change the reading frame of the protein and will make the protein useless