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3. Thermodynamies of protein folding. The native and denatured forms of a protein are generally in...
The native and denatured forms of a protein are generally in equilibrium. For a certain solution...
The native and denatured forms of a protein are generally in equilibrium. For a certain solution of the protein ribonuclease A, in which the total protein concentration is 2.0 x 10^-3 M, the H concentrations of the denatured and native proteins at 50 and 100degreeC are listed below. Determine AS and AH for the folding reaction. [Asinine these quantities are independent of temperature.] Calculate deltaG degree for ribonuclease A folding at 25degreeC. Is this reaction spontaneous at this temperature? What...
For a protein such as ribonuclease, for the process of protein folding under native (non-denaturing) conditions,...
For a protein such as ribonuclease, for the process of protein folding under native (non-denaturing) conditions, Punfolded → Pfolded Select the appropriate description for each of the thermodynamic parameters (for the folding reaction as shown above, from left to right): What is the value of ∆G? What is the value of ∆Sconformational? The value of ∆S hydrophobic? The choices for each question A. positive, favorable for folding B. positive, unfavorable for folding C. negative, favorable for folding D. negative, unfavorable...
5. In an equilibrium denaturation experiment, one makes up a series of different protein solutions, 1,...
5. In an equilibrium denaturation experiment, one makes up a series of different protein solutions, 1, 2, ..., M, having different amounts of urea, a denaturing agent, x1, x2, ..., XM. Then, for each particular solution, having denaturing agent in amount x, one measures the proportion f(x) of native protein molecules and the proportion fo(x) = 1 - fr of denatured protein molecules. Increasing the denaturing agent increases the population of Drelative to N. In the absence of denaturing agent,...
t How are donaturation and varovalo airterent a. Rydrolysis resulte to the formation of water. Hydrolysis...
t How are donaturation and varovalo airterent a. Rydrolysis resulte to the formation of water. Hydrolysis only takes place in the cytopa c. Hydrolysis results in breakingole d. Hydrolysis results in breaking ovalent bo How is folding related to function? • Folding and function are not related.. Proper folding results in propertune 0. Proper folding results in reduced un roper folding results in arratiefunct Molecules that a t folding are called a. Foldons which cellular environment is . Cytoplasm c....
Cyw Ala Ατα Ile Ausn Lou Asp aly Lys Ser Which molecule below is asubunit of...
Cyw Ala Ατα Ile Ausn Lou Asp aly Lys Ser Which molecule below is asubunit of peptide chains a. Acetic acid b. Alanine O. ATP d. Gracil Report the charge on most aqueous aspartie acid molecules at pH-7. Report the approximate p values for the amino acid whose titration curve is shown below (note there may be more slots for answer than answers). A) Value 1 B) Value 2 c) Value 3 c) Value 4 The structure of a rare...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water. 2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA? 3. Which of the following statements about enzymes is true? Select one: a. Enzymes increase the rate of a chemical reaction...
please answer all. thank u Equm U ving reaction for Questions 1 - 3 and answer the questions based upon th sed upon...
please answer all. thank u
Equm U ving reaction for Questions 1 - 3 and answer the questions based upon th sed upon this Consider the following reaction reaction: co (g) + 3 H, (g) + CH (9) + H2O (g) 1. At 25°C. Kega 4.0. What must be Kes 4.0. What must be the coat equilibrium If the rulibrium concentrations on HJE 2.0M, (CH) = 5.OM, and [HO] - 3.OM? Answer: 0.469 M a way to keep track of...
Please answer all, this is for physical chemistry. Thank you T-Mobile 11:14 AM X Problem Set...
Please answer all, this is for physical chemistry. Thank you
T-Mobile 11:14 AM X Problem Set 2_Spring 2018.pdf 1- One gram of liquid bemzene (CH)is bumed in a bomb calorimeter. The temperature before ignition was 20826 C,and the temperature afher the combustion was 25.000 "C. This was an adiabatic calorimeter (constant volame). The heat capacity of the bomb, the water around it, and the contents of the bomb before the combustion was 10,000 JK. Calculate the ??-foe C.H.1) at 298...
29. Which of the following would be synthesized in and processed by the smooth endoplasmic reticulum...
29. Which of the following would be synthesized in and processed by the smooth endoplasmic reticulum and Golgi apparatus? A. lipids and steroids B. DNA polymerase and RNA polymerase C. lysosomal enzymes D. cytoskeletal proteins 30. Which of the following are processed in the Golgi apparatus? A. integral membrane proteins of the plasma membrane B. proteins that are secreted from the cell C. proteins that will be broken down by lysosomes D. all of the above E. none of the...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteina...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
The native and denatured forms of a protein are generally in equilibrium. For a certain solution of the protein ribonuclease A, in which the total protein concentration is 2.0 x 10^-3 M, the H concentrations of the denatured and native proteins at 50 and 100degreeC are listed below. Determine AS and AH for the folding reaction. [Asinine these quantities are independent of temperature.] Calculate deltaG degree for ribonuclease A folding at 25degreeC. Is this reaction spontaneous at this temperature? What...
5. In an equilibrium denaturation experiment, one makes up a series of different protein solutions, 1, 2, ..., M, having different amounts of urea, a denaturing agent, x1, x2, ..., XM. Then, for each particular solution, having denaturing agent in amount x, one measures the proportion f(x) of native protein molecules and the proportion fo(x) = 1 - fr of denatured protein molecules. Increasing the denaturing agent increases the population of Drelative to N. In the absence of denaturing agent,...
t How are donaturation and varovalo airterent a. Rydrolysis resulte to the formation of water. Hydrolysis only takes place in the cytopa c. Hydrolysis results in breakingole d. Hydrolysis results in breaking ovalent bo How is folding related to function? • Folding and function are not related.. Proper folding results in propertune 0. Proper folding results in reduced un roper folding results in arratiefunct Molecules that a t folding are called a. Foldons which cellular environment is . Cytoplasm c....
Cyw Ala Ατα Ile Ausn Lou Asp aly Lys Ser Which molecule below is asubunit of peptide chains a. Acetic acid b. Alanine O. ATP d. Gracil Report the charge on most aqueous aspartie acid molecules at pH-7. Report the approximate p values for the amino acid whose titration curve is shown below (note there may be more slots for answer than answers). A) Value 1 B) Value 2 c) Value 3 c) Value 4 The structure of a rare...
please answer all. thank u
Equm U ving reaction for Questions 1 - 3 and answer the questions based upon th sed upon this Consider the following reaction reaction: co (g) + 3 H, (g) + CH (9) + H2O (g) 1. At 25°C. Kega 4.0. What must be Kes 4.0. What must be the coat equilibrium If the rulibrium concentrations on HJE 2.0M, (CH) = 5.OM, and [HO] - 3.OM? Answer: 0.469 M a way to keep track of...
Please answer all, this is for physical chemistry. Thank you
T-Mobile 11:14 AM X Problem Set 2_Spring 2018.pdf 1- One gram of liquid bemzene (CH)is bumed in a bomb calorimeter. The temperature before ignition was 20826 C,and the temperature afher the combustion was 25.000 "C. This was an adiabatic calorimeter (constant volame). The heat capacity of the bomb, the water around it, and the contents of the bomb before the combustion was 10,000 JK. Calculate the ??-foe C.H.1) at 298...
29. Which of the following would be synthesized in and processed by the smooth endoplasmic reticulum and Golgi apparatus? A. lipids and steroids B. DNA polymerase and RNA polymerase C. lysosomal enzymes D. cytoskeletal proteins 30. Which of the following are processed in the Golgi apparatus? A. integral membrane proteins of the plasma membrane B. proteins that are secreted from the cell C. proteins that will be broken down by lysosomes D. all of the above E. none of the...
1. Describe the functions of the following reagents in extraction of DNA from corn meal: proteinase K; guanidine HCI; SDS 2. Why is the ratio of the OD at 260 and 280 nm used to estimate DNA purity? 3. In one paragraph, summarize basic principles of PCR technique in your own words. List all the reagents you will need to perform a PCR experiment. Does this method tell you what genetic modifications were made? If yes, describe how you can...
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