Question

TVI HOUSE (v) contains U rather than T bases. (vi) may contain stem-loop structure. 6. If you have samples of pure RNA and duplex DNA, how can you tell whether they have any complementary nucleotide sequences?

Answer 1

Answer

Pure RNA and duplex DNA hybridise and from DNA- RNA hybrid. This hybrid with large number of sequences and that are complementary. If the DNA and RNA are complementary then this means the percentage of G (guanine) and C (cytosine) in the RNA is always identical to the percentage of G and C in the DNA (duplex).

So if the RNA- DNA samples form hybrid then they must be complement to each other and this can be identified by calculating G and C percentage in both DNA and RNA (% of G and C must be identical).

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