The enzyme which is purched as the activity of 15000 units/mg ,
So 1mg enzyme had the activity of 15000 units
Thus 0.001mg has the activity of (0.001×15000) units=15 units
So the correct answer is option "B".
An enzyme is purchased with 15,000 units/mg specific activity. What is the activity in 0.001 mg...
The specific activity of a pure enzyme is 6000 μmol substrate transformed.min-1.(mg protein)-1 and its molecular weight is 10,000 Da. Calculate the turnover number of the enzyme in units of μmol substrate transformed.(μmol enzyme)-1.sec-1]? Show all workings including units. Note: A calculator should not be required to answer this question.
If your specific activity of your enzyme is 20 micromoles of product/(min*mg protein), how many micromols of product can be attained in 2 minutes given 2 mg of protein? a. 5 mol b. 20 mol c. 80 mol d. cannot be determined from the data provided in the problem
You have an enzyme solution at a concentration of 5mg/ml. The enzyme activity you determine is 0.1 Units per microliters of undiluted protein. What is the specific activity of the enzyme (U/mg)?
The role of an enzyme in an enzyme-catalyzed reaction is to: Select one: a. make the free-energy change for the reaction more favorable. b. ensure that the product is more stable than the substrate. c. ensure that all the substrate is converted to product. d. increase the rate at which substrate is converted into product. e. do none of the above. The focus of the online practical lesson was the enzyme chymotrypsin. The catalytic mechanism by which chymotrypsin reactions occur:...
D. Dose calibrator un specific activity of 15 mg oaTe pertechnetate that contains 300 mCi in units of MBq/mg? Show your work! MBq/mg.
Enzyme X exhibits maximum activity at pH = 5.25. X shows a sharp decrease in its activity when the pH goes much lower than 4.35. One likely interpretation of this pH activity is that: A) a Glu residue (pKa = 4.25) on the enzyme is involved in the reaction. B) a His residue (pKa = 6.0) on the enzyme is involved in the reaction. C) the enzyme has a metallic cofactor. D) the enzyme is found in gastric secretions. E)...
Briefly: a) Structurally, what kind of compound is an enzyme? b) Enzymes speed up very specific chemical reactions. What feature of the enzyme explains the fact that they only accelerate specific reactions? c) What are two ways that an enzyme be denatured and why do those processes result in the loss of activity of the enzyme? Typed short answer please.
Copy 2 of In tigers, 24 units of enzyme activity are needed for the wild-type (striped) phenotype. Less than 24 units of activity results in a solid colored tiger. Al is the WT allele and produces an enzyme with 16 activity units A2 is the mutant allele and produces an enzyme with 10 activity units Which allele is dominant, and what is the phenotype of a heterozygous tiger? Explain your answer and state whether this is an example of haplosu...
You are asked to measure the amount of β-glucosidase in a commercial enzyme solution containing 20 mg protein/mL of solution. You place 1 mL of this enzyme solution into 9 mL of an aqueous buffer solution containing 20 mM cellobiose. One unit of activity of β-glucosidase is defined as the amount of enzyme which produces 1 µmol of glucose per min in a 65 wt/v% solution of cellobiose at pH 5.0 and at 37°C. Initial rate experiments show that the...
17. How should the specific activity of an enzyme preparation change during its purification? A. Increase B. Decrease C. Remain unchanged D. Unknown; it must be determined for each enzyme individually E. Enzymes cannot be purified 18 18. Which amino acid is being titrated in the figure to the right? A. D B.G C. H D. K E. R NOH The next question concerns the SDS-PAGE figure below. Lane 1 contains an unknown sample. Lane M contains a mixture of...