Homework Answers
Option a is correct. Under such conditions, the operon will be always on. Repressible operon is the one that is constitutively expressed. It is only stopped when the product is enough and it stops it's own transcription by binding to the operator. Tryptophan itself acts as the corepressor and when the repressor cannot bind with the corepressor, it will not be active and thus cannot bind the operator to stop the transcription.
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Question 8 Imagine a repressible operon (such as trp we discussed). Further imagine that a mutation...
There are several possible mutations in the trp operon: trpP -is a mutation in the promoter...
There are several possible mutations in the trp operon:
trpP -is a mutation in the promoter sequence
that prevents RNA polymerase from binding to the promoter and
initiate transcription of the trp operon genes.
trpOcis a mutation in the operator sequence
that prevents the trp repressor protein from binding to
the operator to block transcription of the trp operon
genes.
trpR-is a mutation in the repressor protein
that either prevents repressor protein from being made or produces
a mutant repressor...
P OPERON What happens to transcription at the trp operon when 1. tryptophan levels are low? Why? ...
P OPERON What happens to transcription at the trp operon when 1. tryptophan levels are low? Why? 2. Illustrate it. Include: RNA repressors erase, repressors, and any other molecules needed to show how this worke. GENE TURNED ON -+ + +- Promoter OperatorStructural Genes 3. What happens to transcription at the trp operon when trypto 4. Illustrate it. Include: RNA polymerase, e, repressors and any other molecules needed to show the following GENE TURNED OFF Promoter Operator Structural Genes Circle...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c)...
1) The lac operon is… a) Positive repressible b) Both negative inducible and positive repressible c) Positive inducible d) Negative inducible 2) An activator regulatory protein… a) Is involved in negative regulatory control b) Is a cis acting regulatory element c) Recruits RNA polymerase to the promoter d) Prevents RNA polymerase from binding the promoter 3) For the lac operon, the presence of the substrate (lactose) activates the repressor. True or False? a) true b) false 4) cAMP… a) Activates...
Question 20 1 pts In a case where there is a mutation that prevents the cofactor...
Question 20 1 pts In a case where there is a mutation that prevents the cofactor from binding to the repressor, and the result is that the operon is expressed constitutively. The original type of control in the operon was: negative repressible negative inducible positive repressible positive inducible Previous Nexo
QUESTION 8 The following situations (1-4) involve different types of gene regulation in prokaryotic cells as shown. OFF...
QUESTION 8 The following situations (1-4) involve different types of gene regulation in prokaryotic cells as shown. OFF and ON reter to whether the gene is transcribed or not. Draw clearly-labelled regulatory proteins and effector molecules in each diagram to explain how the regulation works in each case. The first one has been done for you as an example a) (6 marks) Type of Regulation Regulatory protein effector molecule 1 DNA X negative inducible OFF ON 2 negative repressible DNA...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions,...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions, indicate how much (a lot, a little, or none) of the lacZ gene (z-gene) product, beta- galactosidase, is being synthesized. NUTRITIONAL STATUS OF THE BACTERIA +lactose/-glucose +lactose/+glucose -lactose/+glucose genetic status normal (no mutation) mutation a mutation b mutationc important: these are nor the same mutations as the ones in your class handout mutation a: a mutation in the start codon for the lacA (A-gene)...
Page < 2 > of 2 D. The trp operon would be expressed at high levels,...
Page < 2 > of 2 D. The trp operon would be expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance...
1. (1 pt) If a gene is repressible and under positive control, A. Is the regulatory...
1. (1 pt) If a gene is repressible and under positive control, A. Is the regulatory protein an activator or repressor? B. Explain how an effector molecule, which binds to the regulatory protein, alters the regulatory protein’s ability to regulate expression of the gene. 2. (1 pt) Cis and trans are two terms used to describe mutations. A. Explain the molecular difference between these terms. B. indicate which type of mutation (cis or trans) is dominant to wild-type and which...
please help me with the question 15 to 18. Basic structure of an operon Note that...
please help me with the question 15 to 18.
Basic structure of an operon Note that the diagram below is one section of DNA master strend with some areas of DNA labeled in blocks The bracketed area illustrates the basic parts of an operon repressor gene promoter operator structural genes DNA 3 mRNA 5 - 3 repressor protein shown attached to operator #2 Repressor preten "Use purple to color in the repressor gene. The repressor gene codes for a repressor...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
There are several possible mutations in the trp operon:
trpP -is a mutation in the promoter sequence
that prevents RNA polymerase from binding to the promoter and
initiate transcription of the trp operon genes.
trpOcis a mutation in the operator sequence
that prevents the trp repressor protein from binding to
the operator to block transcription of the trp operon
genes.
trpR-is a mutation in the repressor protein
that either prevents repressor protein from being made or produces
a mutant repressor...
P OPERON What happens to transcription at the trp operon when 1. tryptophan levels are low? Why? 2. Illustrate it. Include: RNA repressors erase, repressors, and any other molecules needed to show how this worke. GENE TURNED ON -+ + +- Promoter OperatorStructural Genes 3. What happens to transcription at the trp operon when trypto 4. Illustrate it. Include: RNA polymerase, e, repressors and any other molecules needed to show the following GENE TURNED OFF Promoter Operator Structural Genes Circle...
Question 20 1 pts In a case where there is a mutation that prevents the cofactor from binding to the repressor, and the result is that the operon is expressed constitutively. The original type of control in the operon was: negative repressible negative inducible positive repressible positive inducible Previous Nexo
QUESTION 8 The following situations (1-4) involve different types of gene regulation in prokaryotic cells as shown. OFF and ON reter to whether the gene is transcribed or not. Draw clearly-labelled regulatory proteins and effector molecules in each diagram to explain how the regulation works in each case. The first one has been done for you as an example a) (6 marks) Type of Regulation Regulatory protein effector molecule 1 DNA X negative inducible OFF ON 2 negative repressible DNA...
4. Nutritional status, mutation, and expression of the lac operon. For each of the following conditions, indicate how much (a lot, a little, or none) of the lacZ gene (z-gene) product, beta- galactosidase, is being synthesized. NUTRITIONAL STATUS OF THE BACTERIA +lactose/-glucose +lactose/+glucose -lactose/+glucose genetic status normal (no mutation) mutation a mutation b mutationc important: these are nor the same mutations as the ones in your class handout mutation a: a mutation in the start codon for the lacA (A-gene)...
Page < 2 > of 2 D. The trp operon would be expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance...
please help me with the question 15 to 18.
Basic structure of an operon Note that the diagram below is one section of DNA master strend with some areas of DNA labeled in blocks The bracketed area illustrates the basic parts of an operon repressor gene promoter operator structural genes DNA 3 mRNA 5 - 3 repressor protein shown attached to operator #2 Repressor preten "Use purple to color in the repressor gene. The repressor gene codes for a repressor...
expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance of trp B. The trp operon would be transcribed much less than...
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