Question

LS7A Problem Solving Session 8 (Week 9) 1. Using the space below describe the function of a restriction enzyme using the terms double stranded break, cut site, palindrome, sticky ends, and blunt ends. Explain your description to your LA or neighbor. 2. Draw all the possible fragments generated from the following restriction enzyme cut sites indicated ("*") on the double stranded DNA below. 3- AGCCCT-5 5--TCGC-GA-3' 3. Circle the correct fragments that can be generated from the restriction enzyme cut sites indicated on the double stranded DNA below. Place a square around the fragments that can be combined with the fragments you circles. S'-TTGCAA-3' 3-AACG“TT-5 3-AA-5' 5-CGTT-3' 5'-TT-3' 3'-AACG-5' 5-AA-3' 3-CGTT-5' 5'-TTGC-3' 3'-AA-5' 5'-TTGCAA-3' 3'-TT-5 5'-TG-3' 3'-ACCG-5' 5-CC-3' 3-GGCG-5' 5'-GCAA- 3 3'-TT-5' 5 -GG-3' 3-CGCC-5' 5'-GT-3' 3'-CGCA-5' 5'-TT 3'-AACGTT-5' 3'-TTGC-5 5'-AA-3

Number 1 and 2.

Answer 1

Question 1.
• A restriction enzyme break double stranded DNA by recognising the cut site or palindrome sequence and results in sticky or blunt ends.
• Palindromic sequence is a sequence in double helix of DNA that is the same when read from 5' to 3' on one strand and 5' to 3' on the other complementary strand.
For example -
5' GAATTC 3'
3' CTTAAG 5'.

• Blunt Ends : A straight cut, down through the DNA that results in a flat pair of bases on the ends of the DNA.
Sticky Ends : Staggered ends on a DNA molecule with short, single-stranded overhangs.

Question 2.
Separate DNA strand from given cut ends.

3'- AG*CG CT-s' 5'- TCGCAGA-3' 3- AG tico CGCT-5 AND s'- TCGC A 7 GA-3! STICKч ENDS