What additional genetic experiments would you suggest to verify that this region of cloned DNA contains...
What additional genetic experiments would you suggest to verify that this region of cloned DNA contains a functional promoter?
Select the two correct answers.
|
-Insert the sequence downstream of the coding sequence for a protein whose expression is easy to assay and then introduce that chimeric construct into cells and assay for protein expression. |
|
-Use a known, control promoter to confirm that the protein-coding sequence is correct and that the protein can be detected in the cells used. |
|
-Sequence the DNA and look for promoter elements upstream of the transcription start site which can be found in a start site database. |
| -Insert the sequence upstream of the coding sequence for a protein whose expression is easy to assay and then introduce that chimeric construct into cells and assay for protein expression. |
Homework Answers
4)Insert the sequence upstream of the coding sequence for a protein whose expression is easy to assay and then introduce that chimeric construct into cells and assay for protein expression.
Because if you put your sequence downstream of the coding sequence. It will not get expressed because your gene may have a stop codon. So putting upstream resolve this issue.
Add Answer to:
What additional genetic experiments would you suggest to
verify that this region of cloned DNA contains...
You hypothesize that a DNA sequence located 3.0 kb upstream of the promoter of a gene...
You hypothesize that a DNA sequence located 3.0 kb upstream of the promoter of a gene you are studying contains an enhancer. To test your hypothesis, you use recombinant DNA techniques to insert a DNA fragment containing this DNA sequence into a plasmid also containing a “reporter gene.” Note:A reporter gene encodes for a protein that you can detect when expressed. For example, you could use the lacZ gene that encodes for the enzyme β-Galactosidase as a reporter gene. As...
In female mice, both the glucocorticoid (cortisol) receptor and the estrogen receptor are expressed in liver...
In female mice, both the glucocorticoid (cortisol) receptor and the estrogen receptor are expressed in liver cells (hepatocytes). The control element to which the glucocorticoid receptor binds is a short-strip of DNA that can accommodate the binding of the glucocorticoid receptor dimer. This control element is called a glucocorticoid response element (GRE). It is located1000 bp downstream from the poly-A site for the PEPCK gene. When this gene is activated by cortisol, it helps the hepatocyte to release glucose into...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the DNA immediately upstream of the transcription start site, but expression of the gene can also be affected by control elements. These can be thousands of base pairs upstream of the promoter, grouped in an enhancer. Because the distance and spacing of these control elements make them difficult to identify, scientists begin by deleting sections of DNA that contain possible control elements and measuring the...
You are conducting an experiment identifying enhancers that regulate the expression of a gene that codifies...
You are conducting an experiment identifying enhancers that
regulate the expression of a gene that codifies for a protein that
participates in cell division. The gene is called Mitosis
Regulatory Protein A or MRPA. The complete DNA sequences
for the MRPA promoter and coding region have been
identified. However, it is unknown if MRPA has enhancers
regulating its transcription. To answer this question your lab
first produced a transgenic cell line where GFP has been inserted
as a reporter gene....
The gene machine program shows you what happens when lactose is present in E. coli, and...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
please answer all 6 questions Question 27 3 pts TRBP is a protein important for the...
please answer all 6 questions
Question 27 3 pts TRBP is a protein important for the formation of the RISC complex. Which of the following would you expect in cells with null mutations in TRBP? o Reduced siRNA-mediated mRNA degradation o Increased miRNA-mediated translational repression o Increased deadenylase-mediated mRNA degradation o Reduced proteasome-mediated protein degradation D Question 28 3 pts A protein that binds to the 3' UTR of a VEGF mRNA and promotes deadenylation and uncapping is likely to:...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
You are conducting an experiment identifying enhancers that
regulate the expression of a gene that codifies for a protein that
participates in cell division. The gene is called Mitosis
Regulatory Protein A or MRPA. The complete DNA sequences
for the MRPA promoter and coding region have been
identified. However, it is unknown if MRPA has enhancers
regulating its transcription. To answer this question your lab
first produced a transgenic cell line where GFP has been inserted
as a reporter gene....
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
please answer all 6 questions
Question 27 3 pts TRBP is a protein important for the formation of the RISC complex. Which of the following would you expect in cells with null mutations in TRBP? o Reduced siRNA-mediated mRNA degradation o Increased miRNA-mediated translational repression o Increased deadenylase-mediated mRNA degradation o Reduced proteasome-mediated protein degradation D Question 28 3 pts A protein that binds to the 3' UTR of a VEGF mRNA and promotes deadenylation and uncapping is likely to:...
Most questions answered within 3 hours.
-
Briefly describe:
Investigating and assessing the impact of cost management
quality (CMQ) on the:
(a) effectiveness...
asked 1 hour ago -
Part A:
Use Henry's law to determine the molar solubility of helium at a
pressure of...
asked 1 hour ago -
Can you store the following chemicals in the same storage area:
yes, no and why
a....
asked 3 hours ago -
Hello class,
I found this article to be very interesting and relevant to the
concepts we...
asked 4 hours ago -
Consider the following relational database schema, which
describes the schedule of competition among National Football
(soccer)...
asked 5 hours ago -
Use the answer choices below for the next ____
questions
A) The Heart B) Veins C)...
asked 5 hours ago -
(Java) HELP!
Create a program that will ask the user to enter their first
name and...
asked 5 hours ago -
Suppose that the weight of an newborn fawn is Uniformly
distributed between 2.5 and 4 kg....
asked 6 hours ago -
Java Programming:
Make a Java program with two processes, a producer and a
consumer. If you...
asked 6 hours ago -
A pressure as low as 1 ✕ 10-8 torr can be achieved
using an oil diffusion...
asked 5 hours ago -
Identify the 6 major steps in an operational analysis
and briefly describe what each step is...
asked 5 hours ago -
The nucleus of the atom is composed of subatomic particles, one
of them being the proton...
asked 6 hours ago