Question

________ found in the -10 sequence makes it easier for the DNA to separate.

A. G-C base pairs

B. Template strands

C. Non-template strands

D. A-T base pairs

E. Start codons

9.)Your labmate’s ligation reaction didn’t work. The regular ATP had run out, so he fueled the reaction with dATP from the PCR kit. What advice can you give him?

A. Suggest trying one of the other nucleotides from the PCR kit to drive the ligation reaction.

B. Suggest adding all four nucleotides from the PCR kit.

C. Add heat to the ligation reactions to provide energy, instead of ATP.

D. Remind Sam that dATP is not the same as ATP.

13.)

Answer 1

________ found in the -10 sequence makes it easier for the DNA to separate.
The answer is D. A-T base pairs.

Because A and T binds with Two hydrogen bonds, and requires low amount of energy to cleve these bonds at AT rich bps. So, the DNA unzipping starts at this site.

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