Question

The only charged tRNA that can enter the P site of the ribosome directly (without coming in through the A site) is the initiator tRNA that recognizes the start codon on the mRNA. This requires precise placement of the mRNA on the small subunit of the ribosome so that the initiator tRNA is opposite the P site binding pocket on the large subunit. Compare and contrast how bacteria and eukaryotes achieve this precise positioning.

Answer 1

Prokaryotes ( Bacteria )	Eukaryotes
formylated t-RNA is used for intiation of the translation	formylated or non-formylated t-RNA can be used
three factors are used in translation IF-1, IF-2 and IF-3. ( during initiation )	more than three factors are involved in initiation of translation eIF-1, eIF-2, eIF-3, eIF-4A, eIF4G,eIF5A, eIF5B.
IF-1 bind to the A - site and prevent the initiator t-RNA from binding to the A-site.	eIF1A bind to the A - site and prevent the initiator t-RNA from binding to the A-site.