Homework Answers
Plaques represent the growth of bacteriophages on the E.coli spread on agar plate. Bacteriophages are viruses that infect the bacteria. Viruses insert their DNA into the bacteria and multiply in it. This causes the production of plaques on the agar.
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in bacterophage infection of E. Coli
1. What do the plaques represent and how do they...
E. coli stains that are Hfr: a. are susceptible to infection by bacteriophage. b. are easily...
E. coli stains that are Hfr: a. are susceptible to infection by bacteriophage. b. are easily mutated. c. cannot be made competent. d. are highly recombinant.
A stock of lytic bacteriophages produced 2000 plaques on a plate when 20 μl was plated...
A stock of lytic bacteriophages produced 2000 plaques on a plate when 20 μl was plated with 0.3 ml of E. coli culture. How many plaques would you expect from a 1:1000 dilution of the same stock plated in the same way?
What are the RecBCD proteins of E. coli and what do they do? What is a...
What are the RecBCD proteins of E. coli and what do they do? What is a Chi sequence and what is its function?
4. Strains of Escherichia coli that cause urinary tract infections, known as Uropathogenic E. coli or...
4. Strains of Escherichia coli that cause urinary tract infections, known as Uropathogenic E. coli or UPEC, initiate infection by binding to uroepithelial cells. The E. coli fimbriae FimH binds specifically to D-mannosides on the host uroepithelial cells. A vaccine to stimulate production of neutralizing antibodies that bind to E. coli FimH is in clinical trials. How would this vaccine prevent UPEC infection?
E. coli O157 infection is most commonly associated with consumption of raw or contaminated eggs. a. ...
E. coli O157 infection is most commonly associated with consumption of raw or contaminated eggs. a. True b. False
what are the potential etiologies for infection? how does infection develop?
what are the potential etiologies for infection? how does infection develop?
What are the potential etiologies for infection? How does infection develop?
What are the potential etiologies for infection? How does infection develop?
1. In a phosphate limitation environment, E. coli will be induced to produce alkaline phosphatase. (A)...
1. In a phosphate limitation environment, E. coli will be induced to produce alkaline phosphatase. (A) What is the function of alkaline phosphatase? And how does it help the bacteria to survive in this environment? (B) The phosphate sensing system of E. coli is an example of sensor kinase system. Describe the molecular mechanism of the phosphate sensing system of E. coli using the sensor kinase model. 2. Pseudomonas aeruginosa is used as a bacterial model to study biofilm mechanism....
6. You isolated a E. coli strain from a gastro enteric infection, the physician ask you...
6. You isolated a E. coli strain from a gastro enteric infection, the physician ask you to make a Kirby Bauer test to identify the best antibiotic to eradicate the infection. The results are placed in the table below, Indentify the antibiotics that the bacteria is susceptible and resistant and draw how the inoculated plate would look like Antibiotic name Inhibitory Area and dose (mm) Interpretative Inhibition Zone Resistant (mm) Susceptible or resistant Interpretative Inhibition Zone Susceptible (mm) 215 311...
1. What natural defenses are present in gastrointestinal tract? 2. How does mucus defend against microbial...
1. What natural defenses are present in gastrointestinal tract? 2. How does mucus defend against microbial attack? 3. How do dental caries develop? 4. What is periodontitis and describe the features of each stage? 5. How is STEC different from other E. coli? How did it acquire this ability? 6. How does a person acquire STEC? 7, How are STEC infections treated? 8. How do natural disasters contribute to the transmission of cholera? 9. What does cholera toxin do to...
4. Strains of Escherichia coli that cause urinary tract infections, known as Uropathogenic E. coli or UPEC, initiate infection by binding to uroepithelial cells. The E. coli fimbriae FimH binds specifically to D-mannosides on the host uroepithelial cells. A vaccine to stimulate production of neutralizing antibodies that bind to E. coli FimH is in clinical trials. How would this vaccine prevent UPEC infection?
6. You isolated a E. coli strain from a gastro enteric infection, the physician ask you to make a Kirby Bauer test to identify the best antibiotic to eradicate the infection. The results are placed in the table below, Indentify the antibiotics that the bacteria is susceptible and resistant and draw how the inoculated plate would look like Antibiotic name Inhibitory Area and dose (mm) Interpretative Inhibition Zone Resistant (mm) Susceptible or resistant Interpretative Inhibition Zone Susceptible (mm) 215 311...
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