E. coli stains that are Hfr: a. are susceptible to infection by bacteriophage. b. are easily...
E. coli stains that are Hfr:
a. are susceptible to infection by bacteriophage.
b. are easily mutated.
c. cannot be made competent.
d. are highly recombinant.
Homework Answers
In this case, If you know what Hfr means, you will get the answer. The Hfr means “high frequency recombination” which means that they are highly recombinant. Of of the main features of Hfr cells is that they contain a conjugative plasmid with the fertility factor (Factor F). So, the correct answer is d
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E. coli stains that are Hfr:
a. are susceptible to infection by bacteriophage.
b. are easily...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and...
1. The type of genetic exchange between bacterial cells that can happen between isolated DNA and a live bacterial cell is: a. conjugation b. transduction c. transversion d. transformation 2. An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+...
A T4 bacteriophage transfers a random fragment of DNA from one E. coli cell to another...
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Three Hfr strains of E. coli, each with genotype StrS, lacZ+, A, B, C, D are...
Three Hfr strains of E. coli, each with genotype StrS, lacZ+, A, B, C, D are mated to an F- strain with the genotype StrR, lacZ-, a, b, c, d. Samples of conjugating strains are collected and disrupted every minute for one hour using a blender. Cells are plated under conditions that allow the lacZ+, A, B, C, and D alleles to be identified. The time at which each dominant allele is transferred is shown in the table. lacZ A...
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain...
An Hfr strain of E. coli with genotype a+b+c+d+e+f+ is mated with an F¯ auxotrophic strain with the genotype a¯b¯c¯d¯e¯f¯. Conjugation is stopped at 10 minute intervals and the genotypes of the resulting conjugants are determined. The following results are obtained: after 10 minutes e+ after 20 minutes a+ e+ after 30 minutes a+ b+e+ after 40 minutes a+b+d+e+ after 50 minutes a+b+c+d+e+ after 60 minutes a+b+c+d+e+f+ Which represents the correct order of genes on this bacterial chromosomes? efabcd eabdcf...
You infect E. coli with two strains of T4 bacteriophage with genotypes +B+ and A+C. You...
You infect E. coli with two strains of T4 bacteriophage with genotypes +B+ and A+C. You collect and analyze 1000 plaques, obtaining the following results: ABC = 31 +++ = 36 AB+ = 4 A+C = 410 A++ = 50 +BC = 46 +B+ = 421 ++C = 2 Draw a map with distances for the three genes. Which gene is in the middle? Calculate the interference.
6. You isolated a E. coli strain from a gastro enteric infection, the physician ask you...
6. You isolated a E. coli strain from a gastro enteric infection, the physician ask you to make a Kirby Bauer test to identify the best antibiotic to eradicate the infection. The results are placed in the table below, Indentify the antibiotics that the bacteria is susceptible and resistant and draw how the inoculated plate would look like Antibiotic name Inhibitory Area and dose (mm) Interpretative Inhibition Zone Resistant (mm) Susceptible or resistant Interpretative Inhibition Zone Susceptible (mm) 215 311...
Bacterium ID50 E. coli O157:17 20 1 Legionella pneumophila Shigella 10 Treponema pallidum 57 Which organism...
Bacterium ID50 E. coli O157:17 20 1 Legionella pneumophila Shigella 10 Treponema pallidum 57 Which organism in Table above most easily causes an infection? Select one: O a. E. coli O157:H7 O b. Legionella pneumophila O c. Shigella O d. Treponema pallidum e. The answer cannot be determined based on the information provided.
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks)...
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
Q2 An E. coli culture growing on a lactose supplemented media does not produce any β-galactosidase....
Q2 An E. coli culture growing on a lactose supplemented media does not produce any β-galactosidase. Identify the incorrect statement. a. The E. coli could be a lacZ mutant b. The E. coli may have a mutated LacI protein that cannot bind to the operator c. The E. coli may have a mutated LacI protein that cannot bind to the inducer d. The growth medium may have a high amount of glucose e. The E. coli may have a mutated...
6. You isolated a E. coli strain from a gastro enteric infection, the physician ask you to make a Kirby Bauer test to identify the best antibiotic to eradicate the infection. The results are placed in the table below, Indentify the antibiotics that the bacteria is susceptible and resistant and draw how the inoculated plate would look like Antibiotic name Inhibitory Area and dose (mm) Interpretative Inhibition Zone Resistant (mm) Susceptible or resistant Interpretative Inhibition Zone Susceptible (mm) 215 311...
Bacterium ID50 E. coli O157:17 20 1 Legionella pneumophila Shigella 10 Treponema pallidum 57 Which organism in Table above most easily causes an infection? Select one: O a. E. coli O157:H7 O b. Legionella pneumophila O c. Shigella O d. Treponema pallidum e. The answer cannot be determined based on the information provided.
2. You have performed the following mating experiment using Hfr and F"strains of Escherichia coli:(4 marks) Hfr (asp+ trp+ mal+ StrepS) x F (asp-trp-mal- Strep). Strep = streptomycin, an antibiotic Asp = aspartic acid, an amino acid Trp = tryptophan, an amino acid Mal = maltose, a sugar molecule, can be used by some organisms as an energy source rather than glucose (a) What selective media would you use to score recombinant colonies that have received all of the markers...
2. You have identified a mutant E. coli strain that cannot synthesize histidine (His). To determine the location of the his mutation on the E. coli chromosome, you perform interrupted mating experiments with 5 different Hfr strains. The following chart shows the time of entry (minutes, in parentheses) of the wild-type alleles of the first 5 markers (mutant genes) into the His strain. + + Hfr A. Hfr B Hfr CH Hfr D. Hfr E- his (3) cys (11)- arg...
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