What are the RecBCD proteins of E. coli and what do they do? What is a...
What are the RecBCD proteins of E. coli and what do they do? What is a Chi sequence and what is its function?
Homework Answers
RecBCD proteins are recombination repair protein and they initiate repair of double stranded breaks in DNA caused from ionizing radiation, replication error, endonuclease and oxidative damage. RecBCD has both Helicase and nuclease activity. Chi sequence is a stretch of DNA in the genome of a bacterium near which homologous recombination is most likely to occur. The sequence is GCTGGTGG. Double stranded breaks are modulated by chi sequence.
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What are the RecBCD proteins of E. coli and what do
they do? What is a...
E. coli makes curli fibers on its cell surface that are bacterial amyloid proteins. These curli...
E. coli makes curli fibers on its cell surface that are bacterial amyloid proteins. These curli are used as models to study eukaryotic amyloid proteins in human disease such as Alzheimer's. In E. coli the protein used to secrete curli fibers is CsgG. Using NCBI, search for this protein - "csgG E. coli. K12 W3110" in the protein database, and select the RefSeq protein entry. From the right-hand side of the protein page, select 'Run BLAST'. Select 'Escherichia coli (taxid:562)'...
5 points. When lambda infects an E. coli host what are the first two proteins that...
5 points. When lambda infects an E. coli host what are the first two proteins that are produced? 5 points. In a lambda lysogen, what proteins are produced?
E. coli often comes to mind when reading about proteins and discussing them and it often...
E. coli often comes to mind when reading about proteins and discussing them and it often makes headlines in the news. My challenge for this chapter's discussion topic is this: find a recent (within the last month) news article that involves E. coli and share it with the class. Why does it seem that this bacteria is always in the news? Remember, this chapter is the protein chapter, so why is E. coli so important to proteins and vice versa?...
Expression of a heterologous protein in CHO cells and in an E. coli system produced proteins...
Expression of a heterologous protein in CHO cells and in an E. coli system produced proteins with the same molecular weight on SDS-PAGE. Subsequent in vitro bioactivity studies demonstrated high-level activity with the CHO cell-derived recombinant protein, but no bioactivity with the E. coli derived product. Explain this discrepancy, and propose an experiment to test your hypothesis
B4 (a). Explain the role of following proteins in the replication of E. coli chromosome (10...
B4 (a). Explain the role of following proteins in the replication of E. coli chromosome (10 points) 1) RNase H 2) Gyrase 3) Primase 4) Dna C
3 Briefly discuss some potential problems in expressing eukaryotic proteins in E. coli. (2 marks)
3 Briefly discuss some potential problems in expressing eukaryotic proteins in E. coli. (2 marks)
1. How do prokaryotes conserve energy? 2. How do DNA-binding proteins in prokaryotes regulate genes? 3....
1. How do prokaryotes conserve energy? 2. How do DNA-binding proteins in prokaryotes regulate genes? 3. What is an operon? 4. What is in the lac operon in E. coli? 5. What is the function of the genes in the lac operon of E. coli? 6. What turns the lac operon off? 7. How does a repressor protein turn off the lac operon? 8. How does lactose turn on the lac operon?
One strand of a section of DNA isolated from E. coli reads: 5’GTAGCCTACCCATAGG-3’
One strand of a section of DNA isolated from E. coli reads: 5’GTAGCCTACCCATAGG-3’ (a) Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? (b) What is the amino acid sequence of the proteins. (c) Would the same proteins be made if the other strand of DNA served as template for transcription? Why or why not. (d) If the T underlined in the above sequence was to...
in bacterophage infection of E. Coli 1. What do the plaques represent and how do they...
in bacterophage infection of E. Coli
1. What do the plaques represent and how do they develop?
51. E. coli is being used to study various agents that inhibit protein synthesis. One agent binds...
51. E. coli is being used to study various agents that inhibit protein synthesis. One agent binds the 23S rRNA of the 50S subunit and prevents peptide bond formation. No new proteins are produced. To which antibiotic is this agent likely related? a. chloramphenicol b. streptomycin c. puromycin d. tetracycline e. erythromycin We were unable to transcribe this image57. A protein that affects the expression of many different genes is known as a L 74. One benefit of operons in...
5 points. When lambda infects an E. coli host what are the first two proteins that are produced? 5 points. In a lambda lysogen, what proteins are produced?
B4 (a). Explain the role of following proteins in the replication of E. coli chromosome (10 points) 1) RNase H 2) Gyrase 3) Primase 4) Dna C
1. How do prokaryotes conserve energy? 2. How do DNA-binding proteins in prokaryotes regulate genes? 3. What is an operon? 4. What is in the lac operon in E. coli? 5. What is the function of the genes in the lac operon of E. coli? 6. What turns the lac operon off? 7. How does a repressor protein turn off the lac operon? 8. How does lactose turn on the lac operon?
in bacterophage infection of E. Coli
1. What do the plaques represent and how do they develop?
51. E. coli is being used to study various agents that inhibit protein synthesis. One agent binds the 23S rRNA of the 50S subunit and prevents peptide bond formation. No new proteins are produced. To which antibiotic is this agent likely related? a. chloramphenicol b. streptomycin c. puromycin d. tetracycline e. erythromycin We were unable to transcribe this image57. A protein that affects the expression of many different genes is known as a L 74. One benefit of operons in...
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