3 Briefly discuss some potential problems in expressing eukaryotic proteins in E. coli. (2 marks)
3 Briefly discuss some potential problems in expressing eukaryotic proteins in E. coli. (2 marks)
Homework Answers
One disadvantage of using an organism such as E.coli for expression of eukaryotic genes is that , it is a prokaryotic and therefore lacks the membrane bound nucleus ( and other organelles ) found in eukaryotic cells.
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3 Briefly discuss some potential problems in expressing
eukaryotic proteins in E. coli. (2 marks)
Expressing eukaryotic genes in E. coli: Describe important steps (include various enzymes and their functions) needed...
Expressing eukaryotic genes in E. coli: Describe important steps (include various enzymes and their functions) needed to clone and express proinsulin in E. coli (exclude purification of the protein)
Some simple eukaryotic genes can be transcribed and translated by prokaryotes like E. coli. However, some...
Some simple eukaryotic genes can be transcribed and translated by prokaryotes like E. coli. However, some eukaryotic genes cannot be expressed by prokaryotes. Which of the following might be a reason for this failure? A. Prokaryotes are not able to cut out the introns nor are they able to O splice the exons together from the eukaryotic gene. B. the prokaryotic genetic code is sometimes different from eukaryotic code. C. Eukaryotic genes often have unique nucleotide bases which are not...
Discussion questions Due at the beginning of next weeks' lab 1 How do expression vectors differ...
Discussion questions Due at the beginning of next weeks' lab 1 How do expression vectors differ from vectors used for replicating DNA? (1 mark) 2 What is a fusion protein expression vector? What is the fusion component of pET28a? (2 mark) 3 Briefly discuss some potential problems in expressing eukaryotic proteins in E. coll. (2 marks) 4 Briefly describe a His-tag and how it interacts with its target. (2 mark) 5 How does imidazole act to elute the His-tagged protein...
E. coli makes curli fibers on its cell surface that are bacterial amyloid proteins. These curli...
E. coli makes curli fibers on its cell surface that are bacterial amyloid proteins. These curli are used as models to study eukaryotic amyloid proteins in human disease such as Alzheimer's. In E. coli the protein used to secrete curli fibers is CsgG. Using NCBI, search for this protein - "csgG E. coli. K12 W3110" in the protein database, and select the RefSeq protein entry. From the right-hand side of the protein page, select 'Run BLAST'. Select 'Escherichia coli (taxid:562)'...
Outline the steps required to close a eukaryotic gene using E. coli from mRNA derived from...
Outline the steps required to close a eukaryotic gene using E. coli from mRNA derived from cells expressing the gene. Include how you would amplify the specific gene and how you would select for bacterial cells containing the cloned gene if the gene was inserted into a multiple cloning site within the lacZ gene on a plasmic. If you wanted to later express the gene using a eukaryotic expression vector and promoter how would you ensure the gene was in...
B4 (a). Explain the role of following proteins in the replication of E. coli chromosome (10...
B4 (a). Explain the role of following proteins in the replication of E. coli chromosome (10 points) 1) RNase H 2) Gyrase 3) Primase 4) Dna C
Briefly discuss the cause of foaming and pri ming in steam drums [3 marks) Discuss the driving force behind natural circulation cooling in a power plant that uses subcritical pressures, indicate a pr...
Briefly discuss the cause of foaming and pri ming in steam drums [3 marks) Discuss the driving force behind natural circulation cooling in a power plant that uses subcritical pressures, indicate a problem associated with relatively high subcritical pressures and a method used when this condition is reached or when safety is paramount at supercritical pressures [3 marks] If natural circulation is reduced due to the effects discussed in (b) highlight potential problems in high heat absorbing areas of the...
Review the Human Technology Interface problem and potential improvements. Choose 2 or 3 problems and discuss...
Review the Human Technology Interface problem and potential improvements. Choose 2 or 3 problems and discuss the challenges and potential solutions.
Question #1 While the lecture emphasized the advantages of cloud computing - identify and discuss some...
Question #1 While the lecture emphasized the advantages of cloud computing - identify and discuss some challenges/pitfalls associate in general with cloud computing. Identify and discuss briefly 3 potential problems.
There are some significant differences in how transcription is regulated in prokaryotes like E. coli versus...
There are some significant differences in how transcription is regulated in prokaryotes like E. coli versus eukaryotes. Which of the following statements concerning gene regulation is TRUE only in eukaryotes? (CHOOSE ALL THAT APPLY AND NO INCORRECT ANSWERS) The promoter of a gene can act in a position and distance-independent manner. The genes involved in one process are usually situated next to each other in the genome and are transcribed in one mRNA. The default state of gene transcription is...
Some simple eukaryotic genes can be transcribed and translated by prokaryotes like E. coli. However, some eukaryotic genes cannot be expressed by prokaryotes. Which of the following might be a reason for this failure? A. Prokaryotes are not able to cut out the introns nor are they able to O splice the exons together from the eukaryotic gene. B. the prokaryotic genetic code is sometimes different from eukaryotic code. C. Eukaryotic genes often have unique nucleotide bases which are not...
Discussion questions Due at the beginning of next weeks' lab 1 How do expression vectors differ from vectors used for replicating DNA? (1 mark) 2 What is a fusion protein expression vector? What is the fusion component of pET28a? (2 mark) 3 Briefly discuss some potential problems in expressing eukaryotic proteins in E. coll. (2 marks) 4 Briefly describe a His-tag and how it interacts with its target. (2 mark) 5 How does imidazole act to elute the His-tagged protein...
B4 (a). Explain the role of following proteins in the replication of E. coli chromosome (10 points) 1) RNase H 2) Gyrase 3) Primase 4) Dna C
Briefly discuss the cause of foaming and pri ming in steam drums [3 marks) Discuss the driving force behind natural circulation cooling in a power plant that uses subcritical pressures, indicate a problem associated with relatively high subcritical pressures and a method used when this condition is reached or when safety is paramount at supercritical pressures [3 marks] If natural circulation is reduced due to the effects discussed in (b) highlight potential problems in high heat absorbing areas of the...
Question #1 While the lecture emphasized the advantages of cloud computing - identify and discuss some challenges/pitfalls associate in general with cloud computing. Identify and discuss briefly 3 potential problems.
There are some significant differences in how transcription is regulated in prokaryotes like E. coli versus eukaryotes. Which of the following statements concerning gene regulation is TRUE only in eukaryotes? (CHOOSE ALL THAT APPLY AND NO INCORRECT ANSWERS) The promoter of a gene can act in a position and distance-independent manner. The genes involved in one process are usually situated next to each other in the genome and are transcribed in one mRNA. The default state of gene transcription is...
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