Homework Answers
2. Resolution- it is the shortest distance between the two objects in an image is distinguished and viewed as seperate units.
a the resolving power of human eye is 200 micrometer, light microscope 200nm, electron microscope- o.2nm
b blood cells, mitochondria can be viewed by light microscope, but it does n't have a clear view and proteins are too smaller so all three can be viewed distinctly by electron microscopy.
c advantages of confocal microscope- it is used to eliminate out of focus light in specimens by spatial filtering.
it provides better resolution and it is able to control depth of field & close examination of sample
d
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SCANNING ELECTRON MICROSCOPY |
TRANSMISSION ELECTRON MICROSCOPY |
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It is based on the method of scanning electrons It scans the surface of sample by making the electrons scatter and the machine collects the electron and produce the image and viewed in the monitor It focuses on sample’s surface and its composition. It delivers 3D image It has 2 million level of magnification Resolution-0.4nm |
it is based on transmitting electrons it processes the sample by directing an electron beam and viewed using fluorescent screen. it is used to view interior of the cell it provides only 2D image magnification level is 50 million Resolution—0.5 angstroms |
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what is resolving power of the human eye vs a light mic
2. What is resolution?...
QUESTIONS 1. What is resolution "resolving power"? II. um. What does this 2. The resolving power...
QUESTIONS 1. What is resolution "resolving power"? II. um. What does this 2. The resolving power of the compound light microscope is value mean? 3. Define parfocal, and explain the significance of understanding this concept when using the compound light microscope? 4. What is the total magnification of a compound light microscope? 38 MICROSCOPY and instrumentation Part II: MICROSCOPY: Lab Activity 1
33) Two cells that are 100 am apart can be observed separately by the natural human...
33) Two cells that are 100 am apart can be observed separately by the natural human eye Type: TF ae Rf 2-55 Topic: resolution Skil: Requires Analysis 34) When empty magnification increases, resolution remains the same Type: TF Page Ref: 55GG Topic: resolution Demons 35) The Scanning Electron Microscope magnifies the image of a specimen with a Scanning Objective lens Type: TF Page Ref: 57-67 Topc: electron microscopy Skiit Requires Recollection 36) In a Transmission Electron Microscope, electrons travel right...
3. The resolving power of a microscope depends on the wavelength used. To see an atom, a solution...
3. The resolving power of a microscope depends on the wavelength used. To see an atom, a solution of ~ 1x10-11 m would be required. If electrons were used in an electron microscope, what minimum kinetic energy (in eV) would be required? Compare to the acceleration of the electron beam in a typical high resolution transmission electron microscope (TEM).
3. The resolving power of a microscope depends on the wavelength used. To see an atom, a solution of ~ 1x10-11...
What limits the resolving power of a light microscope? the shortest wavelength of light used to...
What limits the resolving power of a light microscope? the shortest wavelength of light used to illuminate the specimen O the ratio of an object's image to its real size the type of lens that focuses a beam of electrons through the specimen O the type of lens used to magnify the object under study O the type of heavy metal or dye that is used to stain the specimen
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy (EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
Immunocytochemistry is the method of visualization of proteins in cells with the use of antibody that...
Immunocytochemistry is the method of visualization of proteins in cells with the use of antibody that recognize specific molecules. Recently the visualization of these antibody-bound molecules is realized with the imaging of fluorescently-labeled antibodies against these receptors. Which type of microscopy would be used for this study? 0. Confocal microscopy O. Phase-contrast microscopy O. Differential interference contrast microscopy Electron phase-contrast microscopy You are working on a project that involves an imaging of rough endoplasmic reticulum shown here. What microscope was...
need help urgently Question 47 2 pts Neural crest cells are now known to be able...
need help urgently
Question 47 2 pts Neural crest cells are now known to be able to differentiate in to cells. None of the options shown. ectoderm All of the options shown mesoderm endoderm Question 42 2 pts Induced pluripotent stem (iPS) cells can be created by the expression of a set of key genes in most somatic cell types, including cells derived from adult tissues. O require a supply of donor egg cells, as is the case for embryonic...
3. What is the difference between magnification and resolution? Magnification = enlarge What is the purpose...
3. What is the difference between magnification and resolution? Magnification = enlarge What is the purpose of placing immersion oil on the slide? Immersion oil reduce scattering of light or refraction, Explanation @ the Botton! 5. What are the advantages of electron microscopes as compared to light microscopes? Electron has very small wave length Compaled to visible lights 6. Give at least five major differences between prokaryotic and eukaryotic cells. 7. If the ocular magnification is 10X and the oil...
1) Discuss the importance of magnification and resolution in microscopy. How are the magnification and resolution...
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please please help with all of them no thorough explanation needed! 5. Select the most appropriate...
please please help with all of them no thorough explanation needed! 5. Select the most appropriate answer. When using a dichotomous key outside to identify unknown organisms one should A. not remove parts of the plant or disrupt the ecosystem B. be aware of hazards such as ticks and poison ivy C. should write down the steps followed on the key. D. Be aware that your best attempts to identify a specimen might be disrupted by the presence of sexual...
QUESTIONS 1. What is resolution "resolving power"? II. um. What does this 2. The resolving power of the compound light microscope is value mean? 3. Define parfocal, and explain the significance of understanding this concept when using the compound light microscope? 4. What is the total magnification of a compound light microscope? 38 MICROSCOPY and instrumentation Part II: MICROSCOPY: Lab Activity 1
33) Two cells that are 100 am apart can be observed separately by the natural human eye Type: TF ae Rf 2-55 Topic: resolution Skil: Requires Analysis 34) When empty magnification increases, resolution remains the same Type: TF Page Ref: 55GG Topic: resolution Demons 35) The Scanning Electron Microscope magnifies the image of a specimen with a Scanning Objective lens Type: TF Page Ref: 57-67 Topc: electron microscopy Skiit Requires Recollection 36) In a Transmission Electron Microscope, electrons travel right...
3. The resolving power of a microscope depends on the wavelength used. To see an atom, a solution of ~ 1x10-11 m would be required. If electrons were used in an electron microscope, what minimum kinetic energy (in eV) would be required? Compare to the acceleration of the electron beam in a typical high resolution transmission electron microscope (TEM).
3. The resolving power of a microscope depends on the wavelength used. To see an atom, a solution of ~ 1x10-11...
What limits the resolving power of a light microscope? the shortest wavelength of light used to illuminate the specimen O the ratio of an object's image to its real size the type of lens that focuses a beam of electrons through the specimen O the type of lens used to magnify the object under study O the type of heavy metal or dye that is used to stain the specimen
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Immunocytochemistry is the method of visualization of proteins in cells with the use of antibody that recognize specific molecules. Recently the visualization of these antibody-bound molecules is realized with the imaging of fluorescently-labeled antibodies against these receptors. Which type of microscopy would be used for this study? 0. Confocal microscopy O. Phase-contrast microscopy O. Differential interference contrast microscopy Electron phase-contrast microscopy You are working on a project that involves an imaging of rough endoplasmic reticulum shown here. What microscope was...
need help urgently
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3. What is the difference between magnification and resolution? Magnification = enlarge What is the purpose of placing immersion oil on the slide? Immersion oil reduce scattering of light or refraction, Explanation @ the Botton! 5. What are the advantages of electron microscopes as compared to light microscopes? Electron has very small wave length Compaled to visible lights 6. Give at least five major differences between prokaryotic and eukaryotic cells. 7. If the ocular magnification is 10X and the oil...
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