Question

Clouded leopards are a medium sized, endangered species of cat, living in the very wet cloud forests of Central America. Assume that the normal spots (x) are a dominant, sex-linked trait and that dark spots (x") are the recessive trait. Suppose as a conservation biologist, you are involved in a clouded leopard breeding program. One vear you cross a male with dark spots and a female with normal spots The female has four cubs (two male and two female).One each of the male and female cubs have normal spots and one each have dark spots Given the offspring produced, what is the Renotype of the mother? (circle one): x"x" Show your Punnett Square (1 generation) Suppose, a few years later, you breed some of the offspring together (cubs from the first generation). You cross the female cub (from above] that has normal spots with the male that also has normal spots. Show your Punnett Square (2d generationl How many cubs of each genotype will there be (assume 4 total cubs)? xxN. How many of the cubs from this latest cross will have dark spots? What sex will the cubs with dark spots be? xNY: XY:

Answer 1

X^N(Dominant) - Normal spots.
Xⁿ(Recessive) - Dark spots.
Genotype of male with dark spot - XⁿY.
Genotype of female with normal spots - X^NX^N or X^NXⁿ.

Cross XⁿY X X^NXⁿ

Gametes formed Xⁿ and Y X^N and Xⁿ

Next generation

Male/Female	Xn	Y
XN	XNXn(normal spot female)	XNY(normal spot male)
Xn	XnXn(dark spot female)	XnY(dark spot male)

Then, the genotype of mother - X^NXⁿ.

2nd generation:

Cross X^NXⁿ X X^NY

Gametes formed X^N and Xⁿ X^N and Y

Next generation   

Male/Female	XN	Y
XN	XNXN	XNY
Xn	XNXn	XnY

The cubs are present in this generation - X^NX^N, X^NXⁿ, X^NY and XⁿY.

The number of cubs with dark spots = 1.

The sex will the cubs with dark spots be - Male.