Question

3. Number the following prokaryotic promoters in order from strongest (1) to weakest (6).

_____ TTCACACGTGATCTGCGTATCGGTAATAAT

_____ TTGACACGTGATCTGCGTATCGGTTATAAT

_____ TTGACACGGTTATAAT

_____ TTGGCTCGTGATCTGCGTATCGGTTATACG

_____ TATAATCGTGATCTGCGTATCGGTTTGACA

_____ TTGACACGTGATCTATCGATCTGGTAATTGC

Answer 1

2. TTCACA(1)CGTGATCTGCGTATCGGTAATAAT(1) : 2 because only one change at -10, -35 and intact spacer

1. TTGACA(0)CGTGATCTGCGTATCGGTTATAAT(0) : most strong because intact -10, -35 and spacer

5. TTGACACGGTTATAAT (5. deleted spacer but intact -10 and -35)

4. TTGGCT(2)CGTGATCTGCGTATCGGTTATACG(2) : 4 because two changes at -10, -35 and intact spacer

6. TATAAT(6)CGTGATCTGCGTATCGGTTTGACA(4) : 6 because most changes at -10, -35 and intact spacer

3. TTGACA(0)CGTGATCTATCGATCTGGTAATTGC(4): 3 because 4 changes at -10 but intact -35 and spacer