Question

The abundance of mRNA in mammalian cells is measured to be:

a)1% of total RNA content

b)10% of total cell content

c)80% of total RNA content

d)The same as protein content

2. RNAi is used for one of the following purposes.

a) To disrupt a DNA

b) To clone a particular gene

c) To verify the function of a gene

d) To produce a new gene

To successfully express a protein from a eukaryotic expression vector, the following is NOT required.

a) Selectable marker

b) Ribosome binding site

c) Strong eukaryotic promoter

d) Termination sign

Which of the following methods is NOT commonly used for high throughput gene expressionn analysis?

a) Differential Display analysis

b) SAGE

c) DNA microarray

d) Northern blot hybridization

Why would a lab technician choose a bacterial artificial chromosome for cloning a gene, rather than a plasmid?

a)Because the DNA insert is from genomic DNA and not plasmid DNA

b)Because the insert to be cloned is very large.

c)Because bacterial DNA will be used to make many copies of the gene (i.e., clone it).

d)Because the gene insert will eventually be used to transform cells, meaning incorporate the gene into genomic DNA.

Answer 1

Ans 1 b) 10% of total content

mRNA is the messenger RNA . It accounts for approximately 5% of the total RNA content of the mammalian cell. rRNA is present in the maximum amount. It is the ribosomal RNA , which makes approximately 80% of the total RNA content in the cell.