How does the differentiation of alpha and beta hemolysis in members of the genus Streptococcus aid in determining their likely pathogenicity? EXPLAIN IN DETAIL
a veriety of rapid technique or the typing method such as 16rRNA sequencing and PFGE (Pulse field gel electrophoresis, is used for clinical samples.
Multi-locus sequence typing (MLST)
it is used for phylogenetic typing of bacteria and molecular epidemiology.
MLST works on PCR amplification and sequencing of internal fragments of a number (6 or 7) of housekeeping genes pesent on the bacterial chromosome. MLST determine DNA sequence variations in set of essential genes directly and characterises strains by their characteristic allelic profiles.
the technique involve the PCR amplification and then the gene sequencing.
this is one rapid sequencing method.
How does the differentiation of alpha and beta hemolysis in members of the genus Streptococcus aid...
6- Identify a species of Streptococcus that exhibits beta hemolysis, is a group B, and is negative for bile solubility 7- Identify a species of Streptococcus that exhibits alpha hemolysis, is negative for bile esculin hydrolysis, and is not tolerant to 6.5% NaCl. 8- Identify a species of Streptococcus that exhibits beta hemolysis, is negative for the CAMP reaction, and resistant to SXT. 9-identify a group D species that exhibits alpha hemolysis and is tolerant to 6.5% Salt.
Explain the principle of nitrate reduction test? (b) Alpha hemolysis (c) Beta hemolysis (d) Gamma hemolysis.
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