Question

1. Which of these proteins have 3' to 5' exonuclease activity? (A) DNA pol I (B) DNA pol (C) Primase (D) Topoisomerase at kind of mutation is the replacement of a purine-pyrimidine base pair with a pyrimidine- purine base pair, or vice versa? (A) transition mutation (B) insertion (C) transversion mutation (D) missense mutation 3. Whi (A) Tus proteins (B) oriC (C) Ter sites (D) Primosome 4. Which of the following does NOT involve in translesion synthesis in E. coli? (A) DNA Pol I (B) DNA PolI (C) DNA Pol IV (D) DNA Pol V 5. Which technology can be used to detect expression of certain RNA in a tissue? (A) Southern blotting 6. To clone th (A) Restriction enzymes ich of the following acts as a cis element for prokaryotic DNA replication termination? blotting (B) Northem bloting (c) western bloting o) immunoprecipitation e human insulin gene into a plasmid, which of the following is NOT needed? (B) DNA ligase (C) Markers (D) Antibodies 7. Which of these secondary antibodies can be used in a western blotting for a primary antibody produced in rabbit? (A) Rabbit anti-mouse (B) Goat anti-rabbit (C) Goat anti-mouse (D) Rabbit anti-rabbit

Answer 1

1 = (A) DNA polymerase I has 3' to 5' and 5' to 3' exonuclease activity, which is used in editing and proofreading DNA for errors. The 3' to 5' can only remove one mononucleotide at a time.

2 = (A) Transversion mutation is a specific kind of point mutation, in which a single purine is substituted for a pyrimidine or vice versa.

3 = (C) The ter sites are cis acting DNA sequence. The Ter sequences are 23 bp non – palindromic sequences and are binding sites for the Tus protein.

4 = (D)

5 = ( B) it is also called RNA blot. Northern Blotting is a technique used for the study of gene expression. It is done by detection of particular RNA.

6 = (D)

7 = (B) goat anti-rabbit IgG-HRP is a high quality horseradish peroxidase conjugated secondary antibody to be used in western blot.

Hope you have got your answer. Give me positive feedback by clicking on thumbs up so we can serve you better.

THANK YOU.