An organism tests positive for catalase and negative for coagulase. How would this organism appear if streaked onto Mannitol Salt Agar?
According to the question, the organism may be the Staphylococcus aureus and it is positive for the catalase test. It produces the catalase enzyme which converts the hydrogen peroxide to oxygen & water. The catalase test used to distinguish the staphylococci species from the enterococci & streptococci. Most of the nonpathogenic staphylococci are positive for the coagulase test.
Mannitol Salt Agar used to isolate & identify the Staphylococcus aureus. The mannitol salt agar is a differential & selective media. It has the mannitol and phenol red indicator. The Staphylococcus aureus ferments the mannitol and produces an acidic byproduct which turns the phenol red in the agar to yellow. In mannitol salt agar the Staphylococcus aureus produces the colonies with the yellow zone.
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An organism tests positive for catalase and negative for coagulase. How would this organism appear if...
STUDY QUESTIONS: STAPHYLOcocc 1. Why would you perform a coagulase test on a bacterial isolate that tested positive for mannitol salt agar fermentation? 2. Would a catalase positive test always signify the presence of Staphylococci spp.? 3. What is the medical significance of a coagulase positive test result?
Culture site: stitch abscess white, medium sized colony beta hemolysis catalase – positive slide coagulase – positive tube coagulase- positive A disk – negative CAMP test – negative Bile esculin – positive NaCl – positive Optochin disk – negative Bile solubility – negative PYR – negative id the organism
Culture site: impetigo white, small sized translucent, colony beta hemolytic catalase – negative coagulase – negative A disk – positive CAMP test – negative Bile esculin – negative NaCl – negative Optochin disk – negative Bile solubility – negative PYR – positive Id this organism
Microbiology - Gram Positive (ONLY answer) [Q1] Which organism did not ferment mannitol? [Q2] We observe color change on Mueller Hinton agar. (True/ False) [Q3] Which organism was resistant to novobiocin? [Q4] Your organism is catalase negative and produces a black precipiate on bile. What other test would likely be positive?
c. Cellular respiration tests: Catalase.: positive result look like? positive result tell you about the organism? you use for this test? What does a What does a What solution did Oxidase.: What is this test detecting? if an organism is negative? that allows you to determine if an organism is What does it mean What is in the strip positive? Nitrate Reduction: What does this test look for? What does a positive test look like? What does a negative result...
Culture site: wound White to golden, medium sized colony beta hemolytic catalase – positive coagulase latex – positive coagulase tube-negative A disk – negative CAMP test – negative Bile esculin – positive NaCl – positive Optochin disk – negative Bile solubility – negative PYR – positive ID: _____________
uestion 1 Growth of an organism on mannitol salt agar plates indicates that the organism ferments mannitol. tolerates acid. tolerates salt up to 7.5%. is Gram-positive.
Microbiology - Gram Positive (ONLY answer) [Q1] Enterococci and Streptococci are Gram-negative organisms. - True - False [Q2] Which of the following is an important test that should be done first before identifying an unknown to be Staphylococcus or Streptococcus? - Catalase - Coagulase - Blood Agar - A Disc Test [Q3] Which of the following media is used for antibiotic sensitivity testing? - Mannitol - Mueller Hinton - P Disc Test - A Disc Test
Microbiology Matching: a. Mannitol b. Coagulase c. Catalase d. Novobiocin e. Camp f. Bacitracin g. Optochin 1. ___ Genus Streptococcus identified by negative reaction 2. ___ S. aureus is identified by its positive reaction 3. ___ S. saprophyticus is identified by its resistance 4. ___ S. epidermidis is identified by its negative reaction 5. ___ S. pyogenes is identified by sensitivity 6. ___ S. pneumoniae is identified by its sensitivity. 7. ___ S. agalactias is identified by its positive reaction
f. Culture site: stitch abscess White-grey, small sized colony beta hemolytic catalase – -negative coagulase – negative A disk – negative CAMP test – negative Bile esculin – positive NaCl – negative Optochin disk – negative Bile solubility – negative PYR – negative ID: __________