Homework Answers
Dear Student ,
In this circular DNA, when the first cut is made, it opens up the DNA and the second cut divides it into 2 pieces .
So, 2 fragments are obtained at the end. ✅
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til Verizon 11:20 AM Question 2 #2-6 Unanswered If you were to cut the circular piece...
question number 7 purifying plasmid from your cells, you will set up a teaction to cut...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
1. If a restriction enzyme cuts a circular plasmid twice, how many fragments would you see...
1. If a restriction enzyme cuts a circular plasmid twice, how many fragments would you see on the gel? 2. How would you estimate the total number of base pairs in a plasmid by looking at the DNA fragments of the digested plasmid on a gel? 3. If a linear 1kb DNA fragment has a restriction site that is located 50 bp from one end of the plasmid, what would you expect to see if the digested and undigested DNA...
please help if you can 3. You cloned a 20 kb piece of DNA, which contains...
please help if you can
3. You cloned a 20 kb piece of DNA, which contains restriction sites as shown below. 281534 5 B 4 & 5 B = BamHl site, E = EcoRI site, H = Hindill site Numbers above the segments represent the sizes of the regions in kb. Draw and label (in the agarose gel below) the sizes of the fragments you would expect to see after complete digestion of this piece of DNA with the following...
Module 2 homework Laurel Jacqmain- Assignment Score: 20% Resources Hint Check Answer Question 5 of 5...
Module 2 homework Laurel Jacqmain- Assignment Score: 20% Resources Hint Check Answer Question 5 of 5 > A B C D E Restriction mapping of a linear piece of DNA reveals the EcoRI restriction sites. EcoRI site ! EcoRI site 2 2 kb. 4 kb 5 kb 11 kb 6 kb 5 kb 4 kb --3.5 kb 2 kb In each of the scenarios described, DNA is cut by EcoRI and the resulting fragments are separated by gel electrophoresis. The...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
.'ו Optus 11:56 AM Expert Q&A Finite element method QUESTION 4(20 Marks) - concept and calculatio...
finite element method
.'ו Optus 11:56 AM Expert Q&A Finite element method QUESTION 4(20 Marks) - concept and calculation question Consider axial vibration of the circular steel bar shown in Figure 4. The steel bar properties are: p- 7800 kg/m and E-200 GPa. (a)lf the first two natural frequencies are required, sketch the finite element model with clearly labelled element numbers and node numbers, State the element types and the degree of freedoms solved (5 Marks (b)Calculate the stiffiness and...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
i need from a to i please step by step Question 2 (50 points) The template...
i need from a to i please step by step
Question 2 (50 points) The template strand of a buman DNA and a plasmid are given below. The strands are read from left to right. Both DNAs are to be digested by the four base pair cutter restriction enzymes shown in the table Bacteria DNA: 3-ACAGACCATGGAGGCTCCCATGGTTAGCCTGAAGCTTCGA- 3'--CTTIGTGTOCCACCATGGCAGGTACCGAACCTCAACTTTCCTC.-5, Human DNA: Restriction Enzyme Name Ai Bi Ci Di Ei Sequence and type of eut GATC- 3 CTAG 5 T CGA-3 5- G...
am I correct? if not, what's the correct answer and why? thanks Done Quiz 2 20....
am I correct? if not, what's the correct answer and
why? thanks
Done Quiz 2 20. (Problem 10a). For Norma, both nachos and salsa are normal goods. They are also ordinary goods for Norma. The price of nachos rises, but the price of salsa remains unchanged. Can you determine definitively whether she consumes more or fewer nachos? O A. We can say definitively that her consumption of nachos remains unchanged in response to the increase in the price of nachos....
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
please help if you can
3. You cloned a 20 kb piece of DNA, which contains restriction sites as shown below. 281534 5 B 4 & 5 B = BamHl site, E = EcoRI site, H = Hindill site Numbers above the segments represent the sizes of the regions in kb. Draw and label (in the agarose gel below) the sizes of the fragments you would expect to see after complete digestion of this piece of DNA with the following...
Module 2 homework Laurel Jacqmain- Assignment Score: 20% Resources Hint Check Answer Question 5 of 5 > A B C D E Restriction mapping of a linear piece of DNA reveals the EcoRI restriction sites. EcoRI site ! EcoRI site 2 2 kb. 4 kb 5 kb 11 kb 6 kb 5 kb 4 kb --3.5 kb 2 kb In each of the scenarios described, DNA is cut by EcoRI and the resulting fragments are separated by gel electrophoresis. The...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
finite element method
.'ו Optus 11:56 AM Expert Q&A Finite element method QUESTION 4(20 Marks) - concept and calculation question Consider axial vibration of the circular steel bar shown in Figure 4. The steel bar properties are: p- 7800 kg/m and E-200 GPa. (a)lf the first two natural frequencies are required, sketch the finite element model with clearly labelled element numbers and node numbers, State the element types and the degree of freedoms solved (5 Marks (b)Calculate the stiffiness and...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
i need from a to i please step by step
Question 2 (50 points) The template strand of a buman DNA and a plasmid are given below. The strands are read from left to right. Both DNAs are to be digested by the four base pair cutter restriction enzymes shown in the table Bacteria DNA: 3-ACAGACCATGGAGGCTCCCATGGTTAGCCTGAAGCTTCGA- 3'--CTTIGTGTOCCACCATGGCAGGTACCGAACCTCAACTTTCCTC.-5, Human DNA: Restriction Enzyme Name Ai Bi Ci Di Ei Sequence and type of eut GATC- 3 CTAG 5 T CGA-3 5- G...
am I correct? if not, what's the correct answer and
why? thanks
Done Quiz 2 20. (Problem 10a). For Norma, both nachos and salsa are normal goods. They are also ordinary goods for Norma. The price of nachos rises, but the price of salsa remains unchanged. Can you determine definitively whether she consumes more or fewer nachos? O A. We can say definitively that her consumption of nachos remains unchanged in response to the increase in the price of nachos....
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