Question

90 grams H20 EAT 10 grams meat 1 ml 0.1 ml Petri Plates 1 ml of slurry 1 ml 1 ml 1 ml 1 ml 9 ml H20 99 ml H20 9 ml H20 99 ml H20 9 ml H20 0.1 0.1 0.1 1 ml 0.11 ml ml 0.1 ml ml

what are the dilution factors for each plate (A-L)

Answer 1

both plates A and B are plated with the same dilution. when 10 g meat is mixed in 90 ml water, it becomes 10% solution. however, there is no dilution till this point. this can be said as a stock solution or original solution. or since 10 g of meat is dissolved in 100 ml water, this can be said as 10/100 or 10^-1 dilution. therefore, A and B plates would have the same dilutions (10^-1 dilution). however, the volume taken to plate is different.

the first dilution (1ml of the original solution in 9 ml of water) would be 1/10^-1 dilution = 10^-2 dilution. C and D would have 10^-2 dilution factor

second dilution (1 ml of 10^-2 dilution in 99 ml of water) would be 10^-4 dilution. E and F would have 10^-4 dilution factor

third dilution (1 ml of 10^-4 dilution in 9 ml of water) would be 10^-5 dilution. G and H would have 10^-5 dilution factor

fourth dilution (1 ml of 10^-5 dilution in 99 ml of water) would be 10^-7 dilution. I and J would have 10^-7 dilution factor

fifth dilution (1 ml of 10^-7 dilution in 9 ml of water) would be 10^-8 dilution. K and L would have 10^-8 dilution factor