Homework Answers
If we add 1ml sample to 99ml liquid, we dilute our sample to 100 times.
If we add 1ml sample to 9 ml liquid, we dilute our sample to 10 times.
Also, the amount of sample you will get in 1ml of final soulution will be 10 times more than the sample you will expect in 0.1ml of solution or vice versa.
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From the information in the dilution scheme below, give the ive the dilution sequence: 3. Plate...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates,...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
what are the dilution factors for each plate (A-L) 90 grams H20 EAT 10 grams meat...
what are the dilution factors for each plate (A-L)
90 grams H20 EAT 10 grams meat 1 ml 0.1 ml Petri Plates 1 ml of slurry 1 ml 1 ml 1 ml 1 ml 9 ml H20 99 ml H20 9 ml H20 99 ml H20 9 ml H20 0.1 0.1 0.1 1 ml 0.11 ml ml 0.1 ml ml
A student set up a standard plate count on a sample of milk in the following...
A student set up a standard plate count on a sample of milk in the following way: One ml of the milk was diluted into 99 ml of diluent. The first dilution was mixed well. With a new pipette, 0.1 ml of the first dilution was diluted into 0.9 ml of diluent. The second dilution was mixed well, and 0.1 ml of this second dilution was plated on a NA plate. What is the dilution factor of the plate? A.)...
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial cu...
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24...
You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? (2 points) B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8)...
4. Calculate the amount of bacteria in the original sample from the bacterial dilution below (3pt)...
4. Calculate the amount of bacteria in the original sample from the bacterial dilution below (3pt) p.lm 0.1ml> .Im! Sample 0.9ml 9.9ml 0.9ml 10.1ml 10.1ml 10.1ml TNTC 544 5. You have been given 10 ul (0.010ml) of a bacterial stock that contains 7 x 1010 CFU/ml. Diagram a complete dilution scheme that will give you a plate with 100 CFU/ml on it. Be sure to include all steps and remember that you have only 10 ul as an initial sample...
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1....
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions,...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
Meat continued: Plate A Plate B Plate C Gr.Beel, 3 da. 4C, 10-4 dilution Gr.Beel, 3...
Meat continued: Plate A Plate B Plate C Gr.Beel, 3 da. 4C, 10-4 dilution Gr.Beel, 3 da. @ 4C, 10-2 dilution Gr.Beef for 3 da. @ 4C, 10-3 dilution Ground meat was stomached after addition of peptone water. The solution was diluted 10x (10-1), 100x (10-2), 1000x (10-3), 10.000x (10-4). Using the three latter dilutions, 100 ul was spread plated on to TSA and incubated at 37°C. Show dilution series and indicate which dilutions were plated: To estimate cell concentration...
1)How many microliters of original sample are required to produce a final dilution of 10-2 in...
1)How many microliters of original sample are required to produce a final dilution of 10-2 in a total volume of 5 mL? 1 microliter is 10-6 L or 10-3 mL. 2)A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 197 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture?...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
what are the dilution factors for each plate (A-L)
90 grams H20 EAT 10 grams meat 1 ml 0.1 ml Petri Plates 1 ml of slurry 1 ml 1 ml 1 ml 1 ml 9 ml H20 99 ml H20 9 ml H20 99 ml H20 9 ml H20 0.1 0.1 0.1 1 ml 0.11 ml ml 0.1 ml ml
Problem#1 ctice en the following bacterial dilution scheme Name: 1 ml 1 ml 1 ml 1 ml Bacterial culture 4 ml 9 ml 9ml 2ml 0.1 m 1 ml1 m0.1 m 400 CFU 400 CFU 140 CFU 16 CFU Individual tube dilutions Dilution Factor (-Total tube dilutions) Plated dilution factor 1. Explain why plates E and F have the same colony count. 2. Which plate is the countable plate? (30-300 countable range) Calculate the titer of the original culture in...
4. Calculate the amount of bacteria in the original sample from the bacterial dilution below (3pt) p.lm 0.1ml> .Im! Sample 0.9ml 9.9ml 0.9ml 10.1ml 10.1ml 10.1ml TNTC 544 5. You have been given 10 ul (0.010ml) of a bacterial stock that contains 7 x 1010 CFU/ml. Diagram a complete dilution scheme that will give you a plate with 100 CFU/ml on it. Be sure to include all steps and remember that you have only 10 ul as an initial sample...
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
Meat continued: Plate A Plate B Plate C Gr.Beel, 3 da. 4C, 10-4 dilution Gr.Beel, 3 da. @ 4C, 10-2 dilution Gr.Beef for 3 da. @ 4C, 10-3 dilution Ground meat was stomached after addition of peptone water. The solution was diluted 10x (10-1), 100x (10-2), 1000x (10-3), 10.000x (10-4). Using the three latter dilutions, 100 ul was spread plated on to TSA and incubated at 37°C. Show dilution series and indicate which dilutions were plated: To estimate cell concentration...
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