Homework Answers
A = e*C*l
A = absorbance
e = extinction coefficient
C = concentration
l = path length
IF we know all the above set of values
we can find the Concentration from the above formula
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Provided that the extinction coefficient for a compound and path length were known, could an absorbance...
A solution contains 0.00750M of a first compound with an extinction coefficient of 22.4cm−1M−1. A second...
A solution contains 0.00750M of
a first compound with an extinction coefficient of 22.4cm−1M−1. A
second compound is added to the solution at a concentration of
0.0260M. The absorbance of the resulting solution is 0.324 with a
path length of 1.00cm. What is the extinction coefficient for the
second compound in the solution? Round the answer to three
significant figures. Give the answer to three significant
figures.
A solution contains 0.00750 M of a first compound with an extinction coefficient...
Does changes in either concentration or path length result in significant changes in the extinction coefficient....
Does changes in either concentration or path length result in significant changes in the extinction coefficient. Use beers law to explain if possible.
Assuming a path length of 1-cm and a dsDNA extinction coefficient of 0.020 mL·µg-1·cm-1, what is...
Assuming a path length of 1-cm and a dsDNA extinction coefficient of 0.020 mL·µg-1·cm-1, what is the concentration of dsDNA (in nanograms) if A260 was measured to be 0.041?
Absorbance value (A) for a sample prepared was 0.6. The path length (b) of the cuvette...
Absorbance value (A) for a sample prepared was 0.6. The path length (b) of the cuvette used was 1 cm and absorptivity coefficient ε was 0.3 cm2/mg. c is concentration (mg/ml) of the sample in mg/mL. What is the concentration of the sample
Use spectroscoplc methods to measure reaction rates Question A solution contains 0.0125 M of some compound. The ab...
Use spectroscoplc methods to measure reaction rates Question A solution contains 0.0125 M of some compound. The absorbance through a path length of 1.00 cm is 0.364. A second compound with an extinction coefficient of 15.2 cmM is added to the solution, and the absorbance through the path length of 1.00 cm increases to 0.455. What is the concentration of the second compound in the solution? Give the answer to three significant figures. Sorry, that's Incorrect. Try again? M
Please help me answer the following questions: (iv) Calculate the molar extinction coefficient (molar absorptivity) of t...
Please help me answer the following
questions:
(iv) Calculate the molar extinction
coefficient (molar absorptivity) of the maximum absorbance of the
spectrum in Figure 1.
(v) Estimate the wavenumber gap
between the vibrational levels that gives the vibronic structure
apparent in the spectrum of anthracene. Check your answer before
proceeding to answer the next question. (Note: the peaks
are a single vibronic progression so you can average your estimates
of the wavenumber gap.)
5 M compound (2) Figure 1: Absorbance...
What is the concentration of compound Y in an unknown solution if the solution has an...
What is the concentration of compound Y in an unknown solution if the solution has an absorbance of 0.846 in a glass cuvette with a path length of 0.2 cm? The molar absorptivity of compound Y is 54.2 cm-1(mg/ml)-1 under the conditions used for the absorption measurement.
The absorbance (?)(A) of a solution is defined as ?=log10(?0?)A=log10(I0I) where ?0I0 is the incident‑light intensity...
The absorbance (?)(A) of a solution is defined as ?=log10(?0?)A=log10(I0I) where ?0I0 is the incident‑light intensity and ?I is the transmitted‑light intensity. Absorbance is also defined as ?=???A=ϵcl where ?ϵ is the molar absorption coefficient (extinction coefficient) in units of M−1cm−1,M−1cm−1, ?c is the molar concentration, and ?l is path length in centimeters. Daniella prepares a 1 mg/ml1 mg/ml myoglobin solution. The molecular weight of myoglobin is 17.8 kDa.17.8 kDa. Given that the ?ϵ of myoglobin is 15,000 M−1cm−1,15,000 M−1cm−1,...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FDSC Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10" (if your answer is 5.52x10-M then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10-5M (if your answer is 5.52x105M then...
A solution contains 0.00750M of
a first compound with an extinction coefficient of 22.4cm−1M−1. A
second compound is added to the solution at a concentration of
0.0260M. The absorbance of the resulting solution is 0.324 with a
path length of 1.00cm. What is the extinction coefficient for the
second compound in the solution? Round the answer to three
significant figures. Give the answer to three significant
figures.
A solution contains 0.00750 M of a first compound with an extinction coefficient...
Use spectroscoplc methods to measure reaction rates Question A solution contains 0.0125 M of some compound. The absorbance through a path length of 1.00 cm is 0.364. A second compound with an extinction coefficient of 15.2 cmM is added to the solution, and the absorbance through the path length of 1.00 cm increases to 0.455. What is the concentration of the second compound in the solution? Give the answer to three significant figures. Sorry, that's Incorrect. Try again? M
Please help me answer the following
questions:
(iv) Calculate the molar extinction
coefficient (molar absorptivity) of the maximum absorbance of the
spectrum in Figure 1.
(v) Estimate the wavenumber gap
between the vibrational levels that gives the vibronic structure
apparent in the spectrum of anthracene. Check your answer before
proceeding to answer the next question. (Note: the peaks
are a single vibronic progression so you can average your estimates
of the wavenumber gap.)
5 M compound (2) Figure 1: Absorbance...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FDSC Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10" (if your answer is 5.52x10-M then...
Question 1 5 pts The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist collected the following data at 630 nm. She also found that the absorbance of an FD&C Blue Dye #1 solution of unknown concentration was 0.229. Use your graphing calculator to generate a standard curve from this data set, and use it to determine the concentration of the unknown solution. Express your answer in 10-5M (if your answer is 5.52x105M then...
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