Assuming a path length of 1-cm and a dsDNA extinction coefficient of 0.020 mL·µg-1·cm-1, what is...
Assuming a path length of 1-cm and a dsDNA extinction coefficient of 0.020 mL·µg-1·cm-1, what is the concentration of dsDNA (in nanograms) if A260 was measured to be 0.041?
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Assuming a path length of 1-cm and a dsDNA extinction
coefficient of 0.020 mL·µg-1·cm-1, what is...
Provided that the extinction coefficient for a compound and path length were known, could an absorbance...
Provided that the extinction coefficient for a compound and path length were known, could an absorbance measurement be used to determine concentration? Explain your reasoning.
Does changes in either concentration or path length result in significant changes in the extinction coefficient....
Does changes in either concentration or path length result in significant changes in the extinction coefficient. Use beers law to explain if possible.
A solution contains 0.00750M of a first compound with an extinction coefficient of 22.4cm−1M−1. A second...
A solution contains 0.00750M of
a first compound with an extinction coefficient of 22.4cm−1M−1. A
second compound is added to the solution at a concentration of
0.0260M. The absorbance of the resulting solution is 0.324 with a
path length of 1.00cm. What is the extinction coefficient for the
second compound in the solution? Round the answer to three
significant figures. Give the answer to three significant
figures.
A solution contains 0.00750 M of a first compound with an extinction coefficient...
1.) What is the concentration of a solution of PCR primers if the absorption of 260...
1.) What is the concentration of a solution of PCR primers if the absorption of 260 nm (A260) is 0.478 the path length is 1.0 cm and the extinction coefficient (absorptivity) is 185 mM^-1 cm ? a. 0.39 mN b. 3.9 x 10^-3 M c. 2.6 x 10^-3 mmol/L d. 2.6 mmol/L 2.) What is the concentration of a stock solution of PCR primers if the absorption of a diluted solution at 260 nm (A260) is 0.528, the path length...
Suppose a solution of Co(NO3)2 has an extinction coefficient of 5.1L/mol-cm at 505 nm. On graph paper, plot a graph of...
Suppose a solution of Co(NO3)2 has an extinction coefficient of 5.1L/mol-cm at 505 nm. On graph paper, plot a graph of A versus C (mol/L) for solutions of 0.020, 0.040, 0.060, 0.080, and 0.100 M Co(NO3)2 in a 1-cm cell. On the same graph plot, the percent transmittance, or %T, of each solution versus concentration
Suppose a solution of Co(NO3)2 has an extinction coefficient of 5.1 L/mol-cm at 505nm. On the graph provided, plot a gr...
Suppose a solution of Co(NO3)2 has an extinction coefficient of 5.1 L/mol-cm at 505nm. On the graph provided, plot a graph of A versus C (mol/L) for solutions of 0.020, 0.040, 0.060, 0.080, and 0.100 M Co(NO3)2 in a 1.00 cm cell. On the same graph, plot the percent transmittance, %T, of each solution versus concentration.
Question 1 10 pts What is the absorption coefficient, a, of a substance in a 1...
Question 1 10 pts What is the absorption coefficient, a, of a substance in a 1 cm path length cell whose absorbance, A, is 0.102 and concentration, c, is 5.38 x10-5 M? Give your answer to three significant figures. Question 2 8 pts The absorption coefficient, a, is affected by (choose all that apply): Concentration Wavelength being measured Identity of substance in solution Path length
The molar extinction coefficient of a solution of concentration 0.04 M and thickness 10.5 cm is...
The molar extinction coefficient of a solution of concentration 0.04 M and thickness 10.5 cm is 80.08 L mol-1 m-1. Find the value of optical density.
QUESTION 8 Calculate the extinction coefficient in (mg/mL) cm from the following data. It is suggested...
QUESTION 8 Calculate the extinction coefficient in (mg/mL) cm from the following data. It is suggested that you use Excel to do this. Give your answer in 3 significant figures. Assume the path length is 1 cm. (You may want to look at the write-up for LAB 19 -1 c (mg/mL) Abs (e 500 nm) 0.001 0.245 0.002 0.490 0.003 0.735 0.004 0.980 0.005 1.100 0.006 1.200 0.007 1.250
You are a scientist working for a paternity testing center. You spent all Friday isolating dsDNA...
You are a scientist working for a paternity testing center. You spent all Friday isolating dsDNA from 100 hair samples from children and their possible dads. In order to get a quick start on your Southern Blot for Monday, you took absorbance readings for all samples using a spectrophotometer. On Monday morning you realize that you have misplaced some info for 3 of the samples. The bad news is that that spectrophotometer is broken, so you can’t take new measurements...
Provided that the extinction coefficient for a compound and path length were known, could an absorbance measurement be used to determine concentration? Explain your reasoning.
A solution contains 0.00750M of
a first compound with an extinction coefficient of 22.4cm−1M−1. A
second compound is added to the solution at a concentration of
0.0260M. The absorbance of the resulting solution is 0.324 with a
path length of 1.00cm. What is the extinction coefficient for the
second compound in the solution? Round the answer to three
significant figures. Give the answer to three significant
figures.
A solution contains 0.00750 M of a first compound with an extinction coefficient...
Question 1 10 pts What is the absorption coefficient, a, of a substance in a 1 cm path length cell whose absorbance, A, is 0.102 and concentration, c, is 5.38 x10-5 M? Give your answer to three significant figures. Question 2 8 pts The absorption coefficient, a, is affected by (choose all that apply): Concentration Wavelength being measured Identity of substance in solution Path length
QUESTION 8 Calculate the extinction coefficient in (mg/mL) cm from the following data. It is suggested that you use Excel to do this. Give your answer in 3 significant figures. Assume the path length is 1 cm. (You may want to look at the write-up for LAB 19 -1 c (mg/mL) Abs (e 500 nm) 0.001 0.245 0.002 0.490 0.003 0.735 0.004 0.980 0.005 1.100 0.006 1.200 0.007 1.250
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