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For the overall pathway of mannose + PEP converted to mannose-6-P + pyruvate, what is the...
5.The following reaction has a ΔG°'--61.9 kJ/mol at 25°C, Phosphoenolpyruvate (PEP) +H2O Pyruvate + Inorganic Phosphate...
5.The following reaction has a ΔG°'--61.9 kJ/mol at 25°C, Phosphoenolpyruvate (PEP) +H2O Pyruvate + Inorganic Phosphate (Pi) (a) Is the reaction favorable under standard biochemical conditions? Explain your answer. (b) What is the equilibrium constant (K) of this reaction at 25°C? In cells the direct hydrolysis of PEP rarely occurs. Rather, the hydrolysis of PEP and the reverse hydrolysis of ATP are coupled (c) w rite out the coupled reaction and determine its ΔG at 25°C The hydrolysis of ATP...
like me to grade. I vow did the take homeow 1. In glycolysis, the enzyme pyruvate...
like me to grade. I vow did the take homeow 1. In glycolysis, the enzyme pyruvate the enzyme pyruvate kinase catalyzes this reaction phosphoenolpyruvate + ADP - pyruvate + ATP caction using the information provided a. What is the equilibrium constant for below? (R-8.315 J/mol K; T = 298 K) Reaction 1: ATP - ADP +P Reaction 2: phosphoenolpyruvate vale pyruvate + PAG AG"=-30.5 kJ/mol pyruvate + P AGⓇ = -61.9 kJ/mol ntrations are provided below, what is the actual...
Glycolysis activity 1: Metabolic pathway puzzle Try to complete the activity without using the textbook. 1....
Glycolysis activity 1: Metabolic pathway puzzle Try to complete the activity without using the textbook. 1. Slides 4 and 5 show the 10 reactions for glycolysis, but some of the molecules are missing. Draw the missing molecules in the proper place (on the handout). The missing molecules are shown on the next slide, you have to use the clues on the handout and your organic chemistry knowledge to figure out where each molecule belongs. 3. In the red dotted boxes...
1) SHOW WORK 12. During glycolysis, glucose 1-phosphate is converted to fructose 6-phosphate in two successive...
1) SHOW WORK
12. During glycolysis, glucose 1-phosphate is converted to fructose 6-phosphate in two successive reactions: glucose 1-phosphate → glucose 6-phosphate glucose 6-phosphate + fructose 6-phosphate AG'' = -7.1 kJ/mol AG'º = +1.7 kJ/mol 13. The standard free-energy changes for the reactions below are given. phosphocreatine → creatine + Pi ATP → ADP + Pi AG" = 43.0 kJ/mol AG" = -30.5 kJ/mol What is the overall AG' for the following reaction? Iw a dee Hydrolysis of 1 M...
2. The first step in glycolysis is catalyzed by the enzyme herokinase: glucose + ATP glucose-6-P...
2. The first step in glycolysis is catalyzed by the enzyme herokinase: glucose + ATP glucose-6-P + ADP This reaction can be thought of as two coupled reactions (P is phosphate): (1) glucose + P + glucose-6-P (2) ATP + ADP + P For the overall reaction, AG" = -16.7 kJ/mol. For the hydrolysis of ATP (equation 2), AG = -310 kJ/mol. All reactions are performed at 37°C. (a) What is AGº for reaction 1? Iglucose - 6-11 if AG...
Energetics of Phosphorylation of Glucose - making a phospho-ester Use the data in Table to calculate...
Energetics of Phosphorylation of Glucose -
making a phospho-ester
Use the data in Table to calculate ∆G'° for
this reaction:
Glucose + Pi --> Glucose-6-phosphate + H2O
Give the answer in kJ/mole but only write the number in the box,
no units!
Hint: Is the energy going to be positive or
negative? Pay attention to the direction of the reaction!
Reflection: What does this mean? Will this
happen spontaneously in your cells?
Here are screenshots of the Table we are...
QUESTION 12 A eukaryotic cell can use glucose (C6H1206) and hexanoic acid (C6H1402) as fuels for...
QUESTION 12 A eukaryotic cell can use glucose (C6H1206) and hexanoic acid (C6H1402) as fuels for cellular respiration. On the basis of their structural formulas, which of these is more reduced? a hexanoic acid glucose Both are oxidized Cannot be determined QUESTION 13 Which of the substances in question 12 releases less energy per gram on complete combustion to CO2 and H20? Hexanoic acid O glucose Both release the same amount of energy as they are in equilibrium Cannot be...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your work...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: AG 30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate pyruvateP Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 HM, 1.85 mM and 140...
Metabolic Pathway Engineering Problem Set 5 Engineering a Fermentation System: Fermentation of plant matter to produce...
Metabolic Pathway Engineering Problem Set 5 Engineering a Fermentation System: Fermentation of plant matter to produce ethanol for fuel is one potential method for reducing the use of fossil fuels and thus the CO2 emissions that lead to global warming. Many microorganisms can break down cellulose then ferment the glucose to ethanol. However, many potential cellulose sources, including agricultural residues and switchgrass, also contain substantial amounts of arabinose, which is not as easily fermented. Escherichia coli is capable of fermenting...
just the prelab worksheet, no data yet Lab Six: Fermentation Learning Objectives: • Explain the biochemistry...
just the prelab worksheet, no data yet
Lab Six: Fermentation Learning Objectives: • Explain the biochemistry of fermentation, substrates and products, conditions, and purpose for cells • Describe alcoholic fermentation of yeast, naming reactants and products Perform a pre-designed experiment to measure the rate of yeast fermentation of glucose under two different conditions. Propose hypotheses and make predictions based on them. Design and perform a novel experiment to test additional substrates for yeast fermentation using findings of the pre-designed experiment....
5.The following reaction has a ΔG°'--61.9 kJ/mol at 25°C, Phosphoenolpyruvate (PEP) +H2O Pyruvate + Inorganic Phosphate (Pi) (a) Is the reaction favorable under standard biochemical conditions? Explain your answer. (b) What is the equilibrium constant (K) of this reaction at 25°C? In cells the direct hydrolysis of PEP rarely occurs. Rather, the hydrolysis of PEP and the reverse hydrolysis of ATP are coupled (c) w rite out the coupled reaction and determine its ΔG at 25°C The hydrolysis of ATP...
like me to grade. I vow did the take homeow 1. In glycolysis, the enzyme pyruvate the enzyme pyruvate kinase catalyzes this reaction phosphoenolpyruvate + ADP - pyruvate + ATP caction using the information provided a. What is the equilibrium constant for below? (R-8.315 J/mol K; T = 298 K) Reaction 1: ATP - ADP +P Reaction 2: phosphoenolpyruvate vale pyruvate + PAG AG"=-30.5 kJ/mol pyruvate + P AGⓇ = -61.9 kJ/mol ntrations are provided below, what is the actual...
Glycolysis activity 1: Metabolic pathway puzzle Try to complete the activity without using the textbook. 1. Slides 4 and 5 show the 10 reactions for glycolysis, but some of the molecules are missing. Draw the missing molecules in the proper place (on the handout). The missing molecules are shown on the next slide, you have to use the clues on the handout and your organic chemistry knowledge to figure out where each molecule belongs. 3. In the red dotted boxes...
1) SHOW WORK
12. During glycolysis, glucose 1-phosphate is converted to fructose 6-phosphate in two successive reactions: glucose 1-phosphate → glucose 6-phosphate glucose 6-phosphate + fructose 6-phosphate AG'' = -7.1 kJ/mol AG'º = +1.7 kJ/mol 13. The standard free-energy changes for the reactions below are given. phosphocreatine → creatine + Pi ATP → ADP + Pi AG" = 43.0 kJ/mol AG" = -30.5 kJ/mol What is the overall AG' for the following reaction? Iw a dee Hydrolysis of 1 M...
2. The first step in glycolysis is catalyzed by the enzyme herokinase: glucose + ATP glucose-6-P + ADP This reaction can be thought of as two coupled reactions (P is phosphate): (1) glucose + P + glucose-6-P (2) ATP + ADP + P For the overall reaction, AG" = -16.7 kJ/mol. For the hydrolysis of ATP (equation 2), AG = -310 kJ/mol. All reactions are performed at 37°C. (a) What is AGº for reaction 1? Iglucose - 6-11 if AG...
Energetics of Phosphorylation of Glucose -
making a phospho-ester
Use the data in Table to calculate ∆G'° for
this reaction:
Glucose + Pi --> Glucose-6-phosphate + H2O
Give the answer in kJ/mole but only write the number in the box,
no units!
Hint: Is the energy going to be positive or
negative? Pay attention to the direction of the reaction!
Reflection: What does this mean? Will this
happen spontaneously in your cells?
Here are screenshots of the Table we are...
QUESTION 12 A eukaryotic cell can use glucose (C6H1206) and hexanoic acid (C6H1402) as fuels for cellular respiration. On the basis of their structural formulas, which of these is more reduced? a hexanoic acid glucose Both are oxidized Cannot be determined QUESTION 13 Which of the substances in question 12 releases less energy per gram on complete combustion to CO2 and H20? Hexanoic acid O glucose Both release the same amount of energy as they are in equilibrium Cannot be...
Question 1 - Linked Reactions (10 marks) The reaction catalysed by pyruvate kinase is: Phosphoenolpyruvate+ ADPpyruvateATP Keg 3.63 x 105 a) Calculate the AG" for this reaction. Show your working. 3 marks b) The hydrolysis of ATP has following equation: AG 30.5 kJ/mol Calculate the AG" for the following reaction: Phosphoenolpyruvate pyruvateP Show your working. 2 marks c) At 37 °C, the steady-state concentrations of phosphoenolpyruvate, ATP, and ADP have been measured to be 23 HM, 1.85 mM and 140...
just the prelab worksheet, no data yet
Lab Six: Fermentation Learning Objectives: • Explain the biochemistry of fermentation, substrates and products, conditions, and purpose for cells • Describe alcoholic fermentation of yeast, naming reactants and products Perform a pre-designed experiment to measure the rate of yeast fermentation of glucose under two different conditions. Propose hypotheses and make predictions based on them. Design and perform a novel experiment to test additional substrates for yeast fermentation using findings of the pre-designed experiment....
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