here at each cuvette dilution added is by the factor of 10
cuvette 2
Dilution from original is 1:10
then concentration=initial concentration*dilution (dilution=1/fold dilution)
=1M*1/10
=0.1M
cuvette 3
Dilution from original is 1:100
then concentration=initial concentration*dilution (dilution=1/fold dilution)
=1M*1/100
=0.01M
cuvette 4
fold dilution=1000
dilution from original is 1:1000
then concentration=initial concentration*dilution (dilution=1/fold dilution)
=1M*1/1000
=0.001M
cuvette 5
fold dilution=10000
lution from original is 1:10000
then concentration=initial concentration*dilution (dilution=1/fold dilution)
=1M*1/10000
=0.0001M
lete this table if 10-fold dilutions are performed instead of 2-fold dilutions. Cuvette Concentration Dilution fronm...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
#1) What should be the concentration of kanamycin if a serial 2-fold dilution was done starting with tube 1 and ending in tube 9 with an initial concentration of 100 ug/mL tube # 1 2 3 4 5 6 7 8 9 10 [control] Kanamycin concentration (ug/ml) 100 ? ? ? ? ? ? ? ? 0 growth no no no no no yes yes yes yes yes #2) Culture C has a titer of 2.3 x 107 bacteria/mL. Draw...
12. Given a series of 4 dilutions, each 1/5, what is the concentration in tube No. 3 if the original concentration was 100 mg/dL? 13. A 1/2, fourfold serial dilution was performed. The original concentration of the analyte was 1200 μg/dL. What is the concentration of the analyte in the final tube? 7. Calculate the true analyte value given the following information: Analyte diluted using 10 μL into 90 μL diluent; Diluted analyte value = 85 mg/dL.
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
o Calculate the following sample concentrations based upon given dilutions. Final (Diluted) Concentration 1st Dilution 2nd Dilution Concentration Original 1:5 1:4 1:15 1:3 1:3 3 (none) 1:2 1:5 (none) 1:5 167 mg/dL b. 139 mg/dL 15 mg/dL d. 557 mg/dL 23 mg/dL Page 2 of 3
Repeat Part I, using 1M NaOH instead of 1M HCI. Namely, perform five serial dilutions, recording the new concentrations and new pH after each dilution. [H] (OH) рн Data Table Solution Part 1 1M HCI (undiluted) Flask 1 Flask 2 Flask 3 Flask 4 Flask 5 -0.00 0.50 060. 0 90 025 0.625 0.03125 1.0X1o-14 2.0x10-10 40x10-14 8.ox 1014 16x10-13 3.2 x 10-13 1.0rlooly Part II 1M NaOH (undiluted) Flask 1 Flask 2 Flask 3 Flask 4 Flask 5 4.0x...
how do I find molarity of these dilutions? Dilution. Molarity (dilutions) 2:11 4:11 6:11 8:11 10:11
dilutions dilutions What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
1. Make a 10 fold serial dilution of a protein to achieve a final concentration of 10ug/ml You are staring from a 10 mg/ml protein stock. Indicate the amount of sample needed (from previous tube) and the amount of H20 needed. Make your calculations for 100μ1 volume. (0.5pts) 10ul 90μ1 H20 10mg/ml lmg/ml
13. A 1/2, fourfold serial dilution was performed. The original concentration of the analyte was 1200 μg/dL. What is the concentration of the analyte in the final tube? 7. Calculate the true analyte value given the following information: Analyte diluted using 10 μL into 90 μL diluent; Diluted analyte value = 85 mg/dL.