Homework Answers
Proteins with greater molecular size tend to stay at the top of the gel and those with less molecular size tend to pass to the gel bottom.
Hence, the distance travelled by each marker can be given as
Marker size (bp) Distance travelled (cm)
2652 2.8
800 3.5
600 3.9
450 4.0
350 4.1
300 4.3
250 4.4
200 4.5
150 4.7
100 4.8
50 4.9
Add Answer to:
Based on the Gel data complete the table of distance migrated in
cm.
Mode Go E-Gel...
b. Make a TABLE 1 with distance migrated (cm or mm) vs size (in bp) of...
b. Make a TABLE 1 with distance migrated (cm or mm) vs size (in bp) of the 1 kb Ladder standard bands. [See Example) Measure distance migrated for each size standard band on gel. (5 pts) DNA Standard Undigested Pstl+Sac!! Pstl+Stul Pstl+Sacll+Stut Pst! BOS S Kilobases Mass (ng) - 10.0 -80 (bp) 3,300 3,000 2.900 2,700 2,500 0 CC 2,700 2,400 2,000 1,800 1,750 1,800 1,600 1,400 1.300 1,250 1,000 850 800 750 700 650 600 550 -0.5 42 500
Table 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA ...
HELP PLEASE! at least with some examples.
size of dna ladder- 1.8cm
We were unable to transcribe this imageTable 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA band on the gel by interpolating values from your standard curve. There may be multiple bands per lane.For EACH band, identify size (interpolated from the standard curve you constructed), identity, shape, and topology DISTANCE EACH BAND IN THE LANE H AS MIGRATED SIzE OF...
Please help me construct a Ferguson plot for a 12% gel concentration containing Lysozyme the distance...
Please help me construct a Ferguson plot for a 12% gel
concentration containing Lysozyme
the distance for lysozyme is 5.21cm
the distance for BSA is 1.35cm
the distance for RNAse A is 5.58cm
total distance of gel is 7cm
electric field must be approximately E = (100 V)/(20 cm) = 5
V/cm = 500 V/m.
Enzyme MW 02 BSA 66-5K) (2) Lysozyme 14.3 Ra (3) RNase A 13.732 kua -116.0 - 66.2 - 45.0 - 35.0 - 25.0 - -...
1-Indicate in the following table the distance traveled (cm) and the Rf (s) obtained for each...
1-Indicate in the following table the distance traveled
(cm) and the Rf (s) obtained for each sample deposited on the CCM
plate (Use the ruler on the side of the CCM to measure the
distances of the different samples).
Distance traveled by the solvent:
cm
Amidon Fructose Galactose Glucose Lactose Maltose Ribose Saccharose Inconnu 1 Inconnu 2 Inconnu 3 Lait nature Lait lactase Front de migration Sirop d'érable Jus d'orange 5 6 7 8 9 10 11 12 13 14...
Please complete tables for forensic biology lab using the gel image as reference. If you could...
Please complete tables for forensic biology lab using the gel
image as reference.
If you could complete the whole table that would be much
appreciated, however I do not expect anyone to do that much work
for me. If you could just complete a few rows on each one and show
me how you got there, then I'm sure I could complete the rest of it
(I'm just confused on how to calculate the distance in cm)
My teacher gave...
Pvull CAG CTG 6. List three key characteristics of an expression vector 7. Gel electrophoresis ca...
Help with #7 please!!
Pvull CAG CTG 6. List three key characteristics of an expression vector 7. Gel electrophoresis can be used to separate strands of DNA based on size. Tet Nt Smaller pieces migrate more quickly and thus go farther down the gell. Consider the expression vector shown to the right. Sketch a DNA gel that would result from digestion with the following restriction enzymes: PBR328 4907 bps Cm ColE1 ori -EcoRI and Sall Hindill and Sall -EcoRV, Sall,...
М. Table 1: _58cm (20 points) Object Image Magnification Distance Distance p(cm) q(cm) 200 80 190...
М. Table 1: _58cm (20 points) Object Image Magnification Distance Distance p(cm) q(cm) 200 80 190 82 180 84 170 86 160 89.0 150 92 140 96 130 102 120 109 110 119.8 100 134 90 156 Complete Table 1 by calculating the magnifications for each of the object distances. Show work for one of these calculations. 1. Using Excel, or some other graphing program, make a graph of Image Distance vs. Object Distance (q vs. p), then answer the...
Please answer questions 2 and 3 based off my data. Distance Voltage (center) Voltage (edge) cm...
Please answer questions 2 and 3 based off
my data.
Distance Voltage (center) Voltage (edge) cm variable units variable units variable 1 9 9.582 2. 8.5 8.9395 7.2586 3 7.5 7.8411 6.6504 4 6.5 6.7373 6.0478 5 5.5 5.7173 5.4465 6 4.5 4.6754 4.8511 7 3.5 3.7658 4.2657 8 2.5 2.8495 3.6102 9 1.5 1.8063 2.8947 10 0.5 0 2.1416 Voltage (center), Voltage (edge) vs Distance 10 Voltage (center) (units), Voltage (edge) (units) 0 1 2 3 4 5 6...
Enzyme Rf Kda BSA 0.16875 66.5 Lysozyme 0.65125 14.3 RNAse A 0.8225 13.7 Enzyme Cm Log...
Enzyme Rf Kda BSA 0.16875 66.5 Lysozyme 0.65125 14.3 RNAse A 0.8225 13.7 Enzyme Cm Log U BSA 1.35 0.16875 Lysozyme 5.21 0.65125 RNAse A 6.58 0.8225 total distance of gel 8 This is the data for the 12% gel acrylamide Enzyme Rf Kda BSA 0.22779043 66.5 Lysozyme 0.6833713 14.3 RNAse A 0.81548975 13.7 Enzyme Cm Log U BSA 1 0.22779043 Lysozyme 3 0.6833713 RNAse A 3.58 0.81548975 total distance of gel 4.39 This is the data for the 15%...
L= No restriction B=Bam HI E=EcoRI H=Hind III Band 1 27mm 31mm 29mm 29mm Band 2...
L= No restriction
B=Bam HI
E=EcoRI
H=Hind III
Band 1
27mm
31mm
29mm
29mm
Band 2
NA
34mm
41mm
37mm
Band 3
NA
41mm
46mm
43mm
Band 4
NA
43mm
49mm
52mm
Band 5
NA
46mm
57mm
71mm
Band 6
NA
NA
NA
76mm
Above is the actual measurements for the distance in mm. Please
plug this in with the existing chart located above
Gel Electrophoresis lab assignment The following sheets will be used to demonstrate your knowledge of gel...
b. Make a TABLE 1 with distance migrated (cm or mm) vs size (in bp) of the 1 kb Ladder standard bands. [See Example) Measure distance migrated for each size standard band on gel. (5 pts) DNA Standard Undigested Pstl+Sac!! Pstl+Stul Pstl+Sacll+Stut Pst! BOS S Kilobases Mass (ng) - 10.0 -80 (bp) 3,300 3,000 2.900 2,700 2,500 0 CC 2,700 2,400 2,000 1,800 1,750 1,800 1,600 1,400 1.300 1,250 1,000 850 800 750 700 650 600 550 -0.5 42 500
HELP PLEASE! at least with some examples.
size of dna ladder- 1.8cm
We were unable to transcribe this imageTable 8-3. Interpretation of each lane on the gel. For Lanes 1-8, indicate the size of every DNA band on the gel by interpolating values from your standard curve. There may be multiple bands per lane.For EACH band, identify size (interpolated from the standard curve you constructed), identity, shape, and topology DISTANCE EACH BAND IN THE LANE H AS MIGRATED SIzE OF...
Please help me construct a Ferguson plot for a 12% gel
concentration containing Lysozyme
the distance for lysozyme is 5.21cm
the distance for BSA is 1.35cm
the distance for RNAse A is 5.58cm
total distance of gel is 7cm
electric field must be approximately E = (100 V)/(20 cm) = 5
V/cm = 500 V/m.
Enzyme MW 02 BSA 66-5K) (2) Lysozyme 14.3 Ra (3) RNase A 13.732 kua -116.0 - 66.2 - 45.0 - 35.0 - 25.0 - -...
1-Indicate in the following table the distance traveled
(cm) and the Rf (s) obtained for each sample deposited on the CCM
plate (Use the ruler on the side of the CCM to measure the
distances of the different samples).
Distance traveled by the solvent:
cm
Amidon Fructose Galactose Glucose Lactose Maltose Ribose Saccharose Inconnu 1 Inconnu 2 Inconnu 3 Lait nature Lait lactase Front de migration Sirop d'érable Jus d'orange 5 6 7 8 9 10 11 12 13 14...
Please complete tables for forensic biology lab using the gel
image as reference.
If you could complete the whole table that would be much
appreciated, however I do not expect anyone to do that much work
for me. If you could just complete a few rows on each one and show
me how you got there, then I'm sure I could complete the rest of it
(I'm just confused on how to calculate the distance in cm)
My teacher gave...
Help with #7 please!!
Pvull CAG CTG 6. List three key characteristics of an expression vector 7. Gel electrophoresis can be used to separate strands of DNA based on size. Tet Nt Smaller pieces migrate more quickly and thus go farther down the gell. Consider the expression vector shown to the right. Sketch a DNA gel that would result from digestion with the following restriction enzymes: PBR328 4907 bps Cm ColE1 ori -EcoRI and Sall Hindill and Sall -EcoRV, Sall,...
М. Table 1: _58cm (20 points) Object Image Magnification Distance Distance p(cm) q(cm) 200 80 190 82 180 84 170 86 160 89.0 150 92 140 96 130 102 120 109 110 119.8 100 134 90 156 Complete Table 1 by calculating the magnifications for each of the object distances. Show work for one of these calculations. 1. Using Excel, or some other graphing program, make a graph of Image Distance vs. Object Distance (q vs. p), then answer the...
Please answer questions 2 and 3 based off
my data.
Distance Voltage (center) Voltage (edge) cm variable units variable units variable 1 9 9.582 2. 8.5 8.9395 7.2586 3 7.5 7.8411 6.6504 4 6.5 6.7373 6.0478 5 5.5 5.7173 5.4465 6 4.5 4.6754 4.8511 7 3.5 3.7658 4.2657 8 2.5 2.8495 3.6102 9 1.5 1.8063 2.8947 10 0.5 0 2.1416 Voltage (center), Voltage (edge) vs Distance 10 Voltage (center) (units), Voltage (edge) (units) 0 1 2 3 4 5 6...
L= No restriction
B=Bam HI
E=EcoRI
H=Hind III
Band 1
27mm
31mm
29mm
29mm
Band 2
NA
34mm
41mm
37mm
Band 3
NA
41mm
46mm
43mm
Band 4
NA
43mm
49mm
52mm
Band 5
NA
46mm
57mm
71mm
Band 6
NA
NA
NA
76mm
Above is the actual measurements for the distance in mm. Please
plug this in with the existing chart located above
Gel Electrophoresis lab assignment The following sheets will be used to demonstrate your knowledge of gel...
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