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construct a restriction of a circular pvn plasmid, using the following data yor map should indicale,...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
The extracted plasmid DNA is pUC19. Look up the plasmid map and include in your pre-lab as well as your report. a. What is the location of the BamH1 restriction site? Xmn1 restriction site? (sequence location by number). How many of these sites exist each? b. If single digestions were performed (one restriction enzyme) with BamH1 and Xmn1, respectively, how many fragments would form, and what would be the sizes of each of these fragments? c. If a double digestion...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel electrophoresis, the DNA fragments can be separated on a gel, based on their lengths. In order to see the fragments, a stain is typically added to the gel. The size of each fragment can be determined by comparing each one to a DNA molecular weight marker of known size. Below is a map of pBR22 plasmid. The position and base pair number of the...