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2. For the proteins listed below, draw:

a) the order of migration in an isoelectric focusing gel (pH gradient from 3-12). Indicate the location of the electrodes and the direction of the pH gradient.

b) a sketch of a 2D gel electrophoresis experiment for the same set of proteins.

protein MW (kDa pl 13.4 10.6 cyt c 7.0 myoglobin 16.9 14.4 11 lysozyme 4.6 ovalbumin 45.0

Please show work and explain, thank you so much!

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Answer #1

pH 3 negative pH 8 pH 12 positive

pH 3 pH 8 pH 12 MW (kDa) 50 40 ovalbumin 30 20 15 10 myoglobin lysozyme Cyt c

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