2. For the proteins listed below, draw:
a) the order of migration in an isoelectric focusing gel (pH gradient from 3-12). Indicate the location of the electrodes and the direction of the pH gradient.
b) a sketch of a 2D gel electrophoresis experiment for the same set of proteins.
Please show work and explain, thank you so much!
Homework Answers
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2. For the proteins listed below, draw:
a) the order of migration in an isoelectric focusing...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with the following results for isoelectric points and apparent molecular weights: protein A (Mr 110,400; pl 4.60), protein B (Mr 10,100; pl 6.93), protein C (Mr 65,200; pI 7.84), and protein D (M 25,000; pI 8.15) a. After the 2-D separation, which protein will be in the upper left quadrant of the gel. given that e cathode-proximal end of the isoelectric focusing gel was on...
(iln 2. Use the letters given to answer the questions below for full credit Protein name pl A Non...
(iln 2. Use the letters given to answer the questions below for full credit Protein name pl A Nonheme iron-containing fermitin (Pf) B Pynuvate: ferredoxin oxidoreductase, alpha Subunit (PorA) 44613 CGTP-binding proteia (Gtpl) D Co-chaperone (GiroES) E Adhesin-thiol peroxidase (TagD) FI Fumarase (Fund) 19,155 5.49 5.81 40,444 5.53 12,860 6.59 18,161 8.18 50,844 7.29 i) (5 points) Sketch the Isoelectric focusing gel after the separation of above proteins is performed. Clearly label everything needed for full credit. i) (5 points)...
6. You are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with the following results for isoelectric points and apparent molecular weights: protein A (Mr 110,400; pl 4.60), protein B (Mr 10,100; pl 6.93), protein C (Mr 65,200; pI 7.84), and protein D (M 25,000; pI 8.15) a. After the 2-D separation, which protein will be in the upper left quadrant of the gel. given that e cathode-proximal end of the isoelectric focusing gel was on...
(iln 2. Use the letters given to answer the questions below for full credit Protein name pl A Nonheme iron-containing fermitin (Pf) B Pynuvate: ferredoxin oxidoreductase, alpha Subunit (PorA) 44613 CGTP-binding proteia (Gtpl) D Co-chaperone (GiroES) E Adhesin-thiol peroxidase (TagD) FI Fumarase (Fund) 19,155 5.49 5.81 40,444 5.53 12,860 6.59 18,161 8.18 50,844 7.29 i) (5 points) Sketch the Isoelectric focusing gel after the separation of above proteins is performed. Clearly label everything needed for full credit. i) (5 points)...
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