Question

An experiment was conducted in which sections of a eukaryote's DNA were deleted to see the impact on mRNA production. In each case, transcription was measured by looking at the number of transcripts produced after the gene expression was induced. Given the data below, explain why each of the deletions has the impact it does (or does not) (3-4 sentences are probably necessary for a complete answer). For reference, a wild-type cell (no deletions) produced 15,000 transcripts in this experiment.

Answer 1

-1500 through -1200 Since it shows low transcription rate, it means the deletion took place in the regulatory region, like the regions where activators bind, which help in binding of RNA polymerase in correct position on the DNA aarne 350 through -100 -1200 through -900 500 through -350 reg 100 through-1 ts the exact upstream location from the gene sequence to be Since there is no change in this region, it means its between the regulatory regions and the promotor sequence for that gene. That's why deletion caused no change, as no protein binds to this spot. transcribed. It means this is the promoter region, where Polymerase binds and starts the transcription.